We currently perform immunophenotyping for
a number of clinical trials, including CD4/CD8 immunophenotyping for
the Adult Clinical Trials Group (ACTG). Click here to review our
Transplantation Engraftment Monitoring service that combines sorting with chimerism
testing. Please contact Brent Palmer PhD (Brent.Palmer@ucdenver.edu) if you
have additional questions about our services.
The HLA and Flow Cytometry Laboratories
at ClinImmune Labs
and The Molecular Diagnostics Laboratory at Childrens Hospital Colorado, (see brochure), all
located on the Anschutz Medical Campus in Aurora, Colorado, offer integrated
transplant engraftment monitoring services to Hematopoietic Stem Cell
Transplantation (HSCT) Programs. The
laboratories are CLIA, CAP and ASHI accredited.
Sorting: ClinImmune Labs sorts T cells (CD3), myeloid cells
(CD33), and NK cells (CD56) to greater than 96% purity on a FACSAria.
Sorting significantly increases the sensitivity of the chimerism test
and allows the physician to evaluate specific subpopulations. We also customize assays for other requested
Chimerism Testing: The Molecular Diagnostics
Laboratory assays utilizing quantitative polymerase chain reaction/fragment
analysis by capillary electrophoresis. PCR Chimerism is assayed using the Beckman Coulter GeXP GenomeLab
Human STR Primer Set.
Advantages of our One-Stop Service for
Transplant Monitoring Services:
ClinImmune Labs provides high quality HLA testing
for HSCT programs in Colorado and across the nation.
One consolidated requisition form
tailored for each program simplifies the often-cumbersome separate request
processes for sorting and chimerism testing.
Results provided within 3 to 5 days of
Specimens accepted for testing 6 days per
week, Monday through Saturday morning.
Comparative reports provided for
more information about our one stop engraftment monitoring service or a price
quote, please contact Tate Hermanson, (Tate.Hermanson@ucdenver.edu).
Director: Brent Palmer, PhD - Brent.Palmer@ucdenver.edu
Technical Director: Charles Preston Neff, PhD - Charles.Neff@ucdenver.edu
Flow Core Staff:
Garrett Hedlund - Garrett.Hedlund@ucdenver.edu
Sharon Sen - Sharon.Sen@ucdenver.edu