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Welcome to the Integrated Department of Immunology at the University of Colorado - School of Medicine and National Jewish Health.

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Larry Wysocki PhD

Professor of Immunology


 
 

 

 

 

 

 

Education: Ph.D., Harvard University
Email: wysockil@NJHealth.org
Dr Wysocki's National Jewish Health webpage

 

Historically, a major goal of my research program has been to reveal the cellular and molecular basis of antibody formation and the development of memory B cells.  Somatic hypermutation plays a critical role in these processes by altering the sequences of antibody variable (V) genes while B cells respond to foreign immunogen in germinal centers.  Selection pressures normally favor the continued participation of rare B cell clones expressing mutant receptor antibodies that bind immunogen with improved affinity.  As such, the memory B cell repertoire is normally established. 

 

More recently, my laboratory has taken an interest in elucidating the etiology of autoantibody formation in systemic autoimmune diseases such as lupus (SLE).   Although it plays a decisive role in acquired immunity, SHM is a potentially dangerous process and one that we suspect is critical for the generation of such pathological autoantibodies.  Conventional wisdom holds that newly minted B cells emerging from the bone marrow with an autoreactive receptor sometimes escape the self-tolerance mechanisms that normally censor the repertoire and that following activation by self-antigen, such B cells acquire somatic mutations that may improve the affinity with which their receptor antibodies bind self-antigen.  Alternatively, SHM may be playing a much more decisive role by actually creating autoreactive B cell clones de novo from nonautoreactive antecedents.  Our studies to date support the latter of these two alternatives.  As such, we are building a new model to examine self-tolerance in B cells and their escape from self-tolerance at the post-mutational, germinal center stage of activation.

 

In order to participate in autoimmune response, B cells must receive help from ab T cells.  But the origin and antigenic specificity of this help are unknown.    One possibility we are exploring is that somatic hypermutation creates new T cell epitopes within the B cell receptor that enable the autoreactive B cell to obtain T cell help.  This is plausible because B cells not only process and present peptides from foreign immunogen in the context of class II MHC, they also process and present peptides derived from their receptor antibody.  We refer to this idea as the “receptor presentation” hypothesis.    We are looking at this from two perspectives. On the one hand, we are testing the idea that receptor presentation ultimately provides the major avenue of help to pathological autoreactive B cells.  On the other, we are determining how receptor presentation is normally precluded under physiological circumstances.  We assume that specialized tolerance mechanisms must normally regulate this avenue of help, otherwise autoimmunity would be the rule not the exception. To test our ideas, we have developed transgenic lines of mice that carry B cells expressing mutant antibody molecules with defined T cell epitopes and corresponding transgenic lines that carry corresponding ab T cells that react to these epitopes.

 

In a final project, we have developed a unique transgenic model in which B cells express a dual-reactive receptor that binds a self-antigen (ssDNA) with low affinity and a defined hapten called p-azophenylarsonate (Ars).  The dual specificity of this receptor enables us to examine the behavior of these Ars/A1 B cells in immune responses to Ars when it is conjugated to a foreign protein (Ars-carrier).  We have discovered that anergic Ars/A1 B cells strongly inhibit the immune response of normal B cells to Ars and to the foreign carrier protein.  In view of the fact that the wildtype repertoire of B cells contains a substantial fraction of anergic B cells that bind nuclear antigens with low affinity protein, we propose that anergic B cells play a regulatory role in the immune system by inducing tolerance in T cells that are specific for nuclear antigens.  Experiments are underway to test this basic idea.

 

 
  • Guo W, Smith D, Aviszus K, Detanico T, Heiser R and LJ Wysocki.  Somatic hypermutation as a generator of anti nuclear antibodies in a murine model of systemic autoimmunity. J. Exp. Med. 207:2225, 2010.
  • Guth A, Detanico T, Smith D, Tung SK, Bonorino, C. and LJ Wysocki. Spontaneous autoimmunity in mice that carry an Igh V  partial transgene: A Required Arginine in VHCDR3. Lupus 18:299-308, 2009.
  • Aviszus K, Zhang X, and LJ Wysocki.  Silent development of memory progenitor B cells. J Immunol 179:5181-90, 2007.
  • Guo W, DS Smith, A Guth, K Aviszuz and LJ Wysocki,  T cell tolerance to germline-encoded antibody sequences in a lupus-prone mouse.  J. Immunol. 175:2184-90, 2005.
  • Snyder CM, K Aviszus, RA Heiser, DR Tonkin, AM Guth and LJ Wysocki. Activation and tolerance in CD4+ T cells reactive to an immunoglobulin variable region. J Exp Med 200:1-11, 2004.
  • Guth AM, X Zhang, D Smith, T Detanico and LJ Wysocki.  Chromatin specificity of anti-double-stranded DNA antibodies and a role for arginine residues in the third complementarity-determining region of the heavy chain. J Immunol 171:6260-6, 2003.
  • Shapiro GS, MC Ellison and LJ Wysocki. Sequence-specific targeting of two bases on both DNA strands by the somatic hypermutation mechanism.  Mol Immunol 40:287-95, 2003.
  • Benschop RJ, Aviszus K, Zhang X, Manser T, Cambier JC and Wysocki LJ. Activation and anergy in a novel immunoglobulin transgenic mouse that is both hapten-specific and autoreactive. Immunity 14:33-43, 2001.


View of Recent Publications in PubMed