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Gongyi Zhang PhD

Associate Professor of Immunology


 
 

 

 

 

  

Education: Ph.D. 1993, Institute of Biophysics, Chinese Academy of Sciences, Beijing.
Email: zhangg@NJHealth.org
Dr. Zhang's National Jewish Health webpage

The laboratory of Dr. Zhang studies structural and functional relations of macromolecules, which play crucial roles in variety of biological processes. The following are several areas that we are currently focusing on.

1. Structural and functional characterization of JmjC containing histone demethylases.

Covalent modifications of histone proteins, essential regulators of the activity of genes in eukaryotic cells, remodel the chromatin structure via a variety of enzymatic reactions. The reversible processes of some modifications, such as acetylation and deacetylation, are well characterized. Whether methylation and demethylation reversibly contribute to gene regulation, however, remains controversial. Recent studies have shown that methylation and demethylation are universally used to posttranslationally modify histones for the regulation of gene activity. In addition to LSD1, a nuclear amine oxidase homolog, which was found to function as a histone lysine demethylase, it was found that some JmjC domain-containing proteins are histone demethylases. Specifically, in collaboration with Dr. Yang Shi’s group at Harvard university, we found that members of the JMJD2 protein family are histone demethylases that act on trimethyl groups of H3-K9 and H3-K36. Moreover, some members of this family also have activity for dimethyl groups. To understand the relationships between the structures and functions of these proteins, we have determined the structure of the catalytic core of the JMJD2A protein. From this structure, several novel structure features were revealed, such as the novel JmjN domain, the JmjC domain, the C-terminal domain, and a zinc finger motif. These unique structural features create a potential catalytic center. The structure also revealed a characteristic signature motif, which includes structural determinants for cofactors such as Fe(II) and -ketoglutarate, a hallmark of non-heme containing oxygenases.

2. Characterization of Novel signal transduction mechanism through TALL-1 and its cognate receptors.

Trimer is the only functional unit for ligands of TNF family members, and trimer ligands recruit randomly distributed receptors on the membrane so as to trigger the down stream signal transduction. The novel virus-like structures of sTALL-1 with and without its cognate receptors determined in my lab are not consisted with the above common and well accepted theories. The sTALL-1 structure showed that sTALL-1 prefers existing at form of clustering with 20 trimers or 60 monomers through a novel “flap” region in vitro or vivo. Furthermore, the clustering state is the only functional unit. Structures of sTALL-1 with its cognate receptors, BCMA, BAFF-R, and TACI, showed that there are not only novel structural modules in these receptors (such as D2, X2, and others) but also novel interaction modes between ligand and receptors. These structure and interaction novelty underling novel signal transduction mechanism. Furthermore, structural modeling work on TALL-2/APRIL revealed a unique structural basis that distinglishes between sTALL-1 and APRIL for BAFF-R.

3. Structural and functional analysis of a new transcription family members.

Staphylococcus aureus (S. aureus) is a major pathogen in nosocomial bacteremia, generally accounting for ~15-20% of these bacteremic episodes. The expression of microbial virulence factors in S. aureus is a complex process that involves interactions among many gene products. Many of these virulence genes are regulated by global regulatory systems. One of them is the sar (staphylococcal accessory regulator) locus, which not only regulates the agr locus (accessory global regulator, containing protein or peptides, AgrA, AgrB, AgrC, and AgrD), but also directly controls the expression of several putative virulence determinants (e.g. hemolysins, fibrinogen and fibronectin binding proteins). A major transcription factor family plays pivotal roles in the regulation of the sar system, it is called SarA, We have determined several structures of fthe family members, SarR, SarA, and SarS etc. From these four structures, we further classified them in three sub families, SarA motif including SarA and SarR, which functions as homodime, SarS motif, which functions as monomer but contains two homolog SarA motif (as heterodimer), and RAT motif, which functions as homodimer, but contains additional helix bundle at its c-terminal.

  • Hong X, Zang J, White J, Wang C,  Hagman J, Pan C, Zhao R, Murphy R, Dai S, Henson P, Kappler J, Zhang G. (2010). Interaction of JMJD6 with ssRNA.  Proc Natl Acad Sci U S A. Epub 2010 Aug
  • Chen Z, Zang J, Kappler J, Hong X, Crawford F, Wang Q, Lan F, Jiang C, Whetstine J, Dai S, Hansen K, Shi Y, Gongyi Zhang. (2007). Structural basis of the recognition of a methylated histone tail by JMJD2A. Proc Natl Acad Sci U S A. 2007 Jun 13; [Epub ahead of print].
  • Chen Z, Zang J, Whetstine J, Hong X, Davrazou F, Kutateladze TG, Simpson M, Mao Q, Pan CH, Dai S, Hagman J, Hansen K, Shi Y, Zhang G. Structural insights into histone demethylation by JMJD2 family members. Cell. 2006 May 19;125(4):691-702. Epub 2006 May 4.
  • Whetstine JR, Nottke A, Lan F, Huarte M, Smolikov S, Chen Z, Spooner E, Li E, Zhang G, Colaiacovo M, Shi Y. Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases. Cell. 2006 May 5;125(3):467-81. Epub 2006 Apr 6.
  • Liu Y, Manna AC, Pan CH, Kriksunov IA, Thiel DJ, Cheung AL, Zhang G. Structural and function analyses of the global regulatory protein SarA from Staphylococcus aureus. Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2392-7. Epub 2006 Feb 2.
  • Zhang G. Tumor necrosis factor family ligand-receptor binding. Curr Opin Struct Biol. 2004 Apr;14(2):154-60.
  • Liu Y, Hong X, Kappler J, Jiang L, Zhang R, Xu L, Pan CH, Martin WE, Murphy RC, Shu HB, Dai S, Zhang G. Ligand-receptor binding revealed by the TNF family member TALL-1. Nature. 2003 May 1;423(6935):49-56.
  • Liu Y, Xu L, Opalka N, Kappler J, Shu HB, Zhang G. Crystal structure of sTALL-1 reveals a virus-like assembly of TNF family ligands. Cell. 2002 Feb 8;108(3):383-94.
  • Cheung AL, Zhang. Are the structures of SarA and SarR similar? Trends Microbiol. 2001 Dec;9(12):570-3.
  • Liu Y, Manna A, Li R, Martin WE, Murphy RC, Cheung AL, Zhang G. Crystal structure of the SarR protein from Staphylococcus aureus. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6877-82. Epub 2001 May 29.
  • Zhang G, Campbell EA, Minakhin L, Richter C, Severinov K, Darst SA. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution. Cell. 1999 Sep 17;98(6):811-24.
  • Zhang G, Darst SA. Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain. Science. 1998 Jul 10;281(5374):262-6.
  • Zhang G, Liu Y, Ruoho AE, Hurley JH. Structure of the adenylyl cyclase catalytic core. Nature. 1997 Mar 20;386(6622):247-53.
  • Zhang G, Kazanietz MG, Blumberg PM, Hurley JH. Crystal structure of the cys2 activator-binding domain of protein kinase C delta in complex with phorbol ester. Cell. 1995 Jun 16;81(6):917-24.


View of Recent Publications in PubMed