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University of Colorado Department of Biochemistry and Molecular Genetics

Biochemistry and Molecular Genetics


  1. O’Bleness, M.S., Searles, V., Varki, A., Gagneux, P., and Sikela, J.M. (2012). Evolution of genetic and genomic features unique to the human lineage. Nat. Rev. Genet., 13, 853-866.
  2. Dumas, L.J., O’Bleness, M.S., Davis, J.M., Dickens, C.M., Anderson, N., Keeney, J.G., Jackson, J., Sikela, M., Raznahan, A., Giedd, J., et al. (2012). DUF1220-domain copy number implicated in human brain-size pathology and evolution. Am. J. Hum. Genet. 91, 444–454.
  3. O’Bleness, M.S., Dickens, C.M., Dumas, L.J., Kehrer-Sawatzki, H., Wyckoff, G.J., Sikela, J.M. (2012). Evolutionary history and genome organization DUF1220 protein domains. G3 (Bethesda) 2, 977–986.
  4. Karimpour-Fard, A., Dumas, L., Phang, T., Sikela, J.M., and Hunter, L.E. (2010). A survey of analysis software for array-comparative genomic hybridization studies to detect copy number variation. Hum. Genomics 4, 421–427.
  5. Coors, ME., Glover, JJ., Juengst, ET., and Sikela, J.M. The ethics of using transgenic non-human primates to study what makes us human. Nat Rev Genet., 11:658-662, 2010.
  6. Karimpour-Fard, A., Dumas, L., Phang, T., Sikela, J.M., and Hunter, L.E. A survey of analysis software for array-comparative genomic hybridization studies to detect copy number variation. Human Genomics 4(6):421-427, 2010.
  7. Sikela, J.M. (2010), "Gene copy number variation in human and primate evolution," in Scherer, S. (ed.), Copy Number Variation: The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at
  8. Dumas, L and Sikela, J.M. DUF1220 Domains, Cognitive Disease, and Human Brain Evolution. Cold Spring Harbor Symp. Quant. Biol. October, 2009.
  9. Dumas, L., Kim, Y.H., Karimpour-Fard, A., Cox, M., Hopkins, J., Pollack, J.R., and Sikela, J.M.: Gene copy number variation spanning 60 million years of human and primate evolution. Genome Research, 17:1266-1277, 2007.
  10. Rhesus Macaque Genome Sequencing and Analysis Consortium. Evolutionary and Biomedical Insights from the Rhesus Macaque Genome. Science 316: 222-234, 2007.
  11. Babcock, M., Yatsenko, S., Hopkins, J., Brenton, M., Cao, Q., de Jong, P., Stankiewicz, P., Lupski, J.R., Sikela, J.M., Morrow, B.E.: Hominoid lineage specific amplification of low-copy repeats on 22q11.2 (LCR22s) associated with velo-cardio-facial/DiGeorge syndrome. Hum Mol Genet 16: 2560-2571, 2007.
  12. Popesco M.C., MacLaren E.J., Hopkins J., Dumas L., Cox M., Meltesen L., McGavran L., Wyckoff G.J., and Sikela J.M.. Human Lineage-Specific Amplification, Selection, and Neuronal Expression of DUF1220 Domains. Science 313: 1304-1307, 2006. (Abstract)  (Full Text Article)
  13. Sikela J.M. The Jewels of Our Genome: The Search for the Genomic Changes Underlying the Evolutionarily Unique Capacities of the Human Brain. PLoS Genet 2(5): e80, 2006.
  14. Sikela, J.M., Maclaren, E.J., Kim, Y., Karimpour-Fard, A., Cai, W.-W., Pollack, J., Hitzemann, R., Belknap, J., McWeeney, S., Kerns, R.T., et al. (2006). DNA microarray and proteomic strategies for understanding alcohol action. Alcohol. Clin. Exp. Res. 30, 700–708.
  15. MacLaren, E.J., Bennett, B., Johnson, T.E., and Sikela, J.M. (2006). Expression profiling identifies novel candidate genes for ethanol sensitivity QTLs. Mamm. Genome 17, 147–156.
  16. Moniaux, N., Nemos, C., Schmied, B.M., Chauhan, S.C., Deb, S., Morikane, K., Choudhury, A., Vanlith, M., Sutherlin, M., Sikela, J.M., et al. (2006). The human homologue of the RNA polymerase II-associated factor 1 (hPaf1), localized on the 19q13 amplicon, is associated with tumorigenesis. Oncogene 25, 3247–3257.
  17. Haughey, H.M., Kaiser, A.L., Johnson, T.E., Bennett, B., Sikela, J.M., and Zahniser, N.R. (2005). The norepinephrine transporter: A candidate gene for initial ethanol sensitivity in inbred long-sleep and short-sleep mice. Alcohol. Clin. Exp. Res. 29, 1759-1768.
  18. MacLaren, E.J., and Sikela, J.M. (2005). Cerebellar gene expression profiling and eQTL analysis in inbred mouse strains selected for ethanol sensitivity. Alcohol. Clin. Exp. Res. 29, 1568–1579.
  19. Kerns, R.T., Ravindranathan, A., Hassan, S., Cage, M.P., York, T., Sikela, J.M., Williams, R.W., and Miles, M.F. (2005). Ethanol-responsive brain region expression networks: implications for behavioral responses to acute ethanol in DBA/2J versus C57BL/6J mice. J. Neurosci. 25, 2255–2266.
  20. Fortna, A., Kim, Y., MacLaren, E., Marshall, K., Hahn, G., Meltesen, L., Brenton, M., Hink, R., Burgers, S., Hernandez-Boussard, T., Karimpour-Fard, A., Glueck, D., McGavran, L., Berry, R., Pollack, J.R. and Sikela, J.M. Lineage-specific gene duplication and loss in human and great ape evolution. PLoS Biology Jul 2(7):E207, 2004 .
  21. Hitzemann, R., Malmanger, B., Reed, C., Lawler, M., Hitzemann, B., Coulombe, S., Buck, K., Rademacher, B., Walter, N., Polyakov, Y., Sikela, J., Williams, R.W., Flint, J., and Talbot, C. A Strategy for the Integration of QTL, Gene Expression and Sequence Analyses. Mammalian Genome 14: 733-747, 2003.
  22. Abiola, O., Angel, J.M., Avner, P., Bachmanov, A.A., Sikela, J.M., and Flaherty, L. (79 authors): A community's view on the nature and identification of quantitative trait loci (QTLs). Nature Reviews Genetics 4:910-916, 2003.
  23. Hink, R., Hokanson, J., Shah, I., Long, J., Goldman, D., and Sikela, J.M.: Investigation of DUSP8 and CALCA in alcohol dependence. Addiction Biology 8:301-309, 2003.
  24. Saito, M., Ehringer, M.A., Toth, R., Oros, M., Szakall,I., Sikela, J.M., and Vadasz, C.: Variants of μ opioid receptor gene and mRNA in alcohol-preferring and -avoiding mice. Alcohol 29:39-49, 2003.
  25. Sikela, J.M.: Invited Review of "The Shattered Self", Perspectives in Biology and Medicine, 46 2003.
  26. Marshall, K.E., Godden, E.L., Yang, F., Burgers, S., Buck, K.J., and Sikela, J.M.: In silico discovery of gene coding variants in murine QTLs using strain-specific genome sequence databases. Genome Biology, 3:0078.1- 0078.9, 2002.
  27. Ehringer, M., and Sikela, J.M.: Genomic Approaches to the Genetics of Alcoholism. Alcohol Research and Health, 26:181-192, 2002.
  28. Ehringer, M.A., Thompson, J., Conroy, O., Yang, F., Hink, R., Bennett, B., Johnson, T.E., and Sikela, J.M.: Fine-mapping of polymorphic alcohol-related QTL candidate genes using interval-specific congenic recombinant mice. Alcoholism: Clinical & Experimental Research 26:1603-1608, 2002.
  29. Ehringer, M.A., Thompson, J., Conroy, O., Goldman, D., Smith, T.L., Schuckit, M.A., and Sikela, J.M.: Human alcoholism studies of genes identified through mouse QTL analysis. Addiction Biol. 7:365-371, 2002.
  30. Kirstein, S.L., Davidson, K., Ehringer, M.A., Sikela, J.M., Erwin, V.G., and Tabakoff, B.: Quantitative trait loci affecting initial sensitivity and acute functional tolerance to ethanol-induced ataxia and brain cAMP signaling in BXD RI mice. Journal of Pharmacology and Experimental Therapeutics 302: 1238-1245, 2002.
  31. Xu, Y., Demarest, K., Hitzemann, R., and Sikela, J.M.: Gene coding variation in Cas1 between the C57BL/6J and DBA/2J Inbred Mouse Strains: Linkage to a QTL for ethanol-induced locomotor activation. Alcoholism: Clinical and Experimental Research 26:1-7, 2002.
  32. Ehringer, M.E., Thompson, J.T., Conroy, O., Yang, F., Hink, R., Burgers, S., Bennett, B., Johnson, T.E., and Sikela, J.M.: High throughput comparative sequence identification of gene coding variants within alcohol related QTLs. Mammalian Genome 12:657-663, 2001.
  33. Xu, Y., Ehringer, M.E., Yang, F., and Sikela, J.M.: Comparison of global brain gene expression profiles between ILS and ISS mice by high-density gene array hybridization. Alcoholism: Clinical and Experimental Research 25:810-818, 2001.
  34. McConkey, E.H. & Varki, A. Cosignatories: Allman, J., Benirschke, K., Crick, F., Deacon, T.W., de Waal, F., Dugaiczyk, A., Gagneux, P., Goodman, M., Grossman, L.I., Gumucio, D., Insel, T., Kidd, K.K., King, M.-C., Krauter, K., Kucherkapati, R., Motulsky, A.G., Nelson, D., Oefner, P., Palade, G., Ruvolo, M., Ryder, O.A., Sikela, J., Stewart, C.-B., Stone, A. & Woodruff, D.: A primate genome project deserves high priority. Science 289:1295-1296, 2000.
  35. McConkey, E.H., Fouts, R., Goodman, M., Nelson, D., Penny, D., Ruvolo, M., Sikela, J.M., Stewart, C.B., Varki, A., and Wise, S.: Proposal for a Human Genome Evolution Project. Molecular Phylogenetics and Evolution 15:1-4, 2000.
  36. Bateman, J.B., Geyer, D., Flodman, P., Johannes, M., Walter, N.W., Sikela, J., Moreira, A.T., Clancy, K. and Spence, M.A.: A new betaA1-crystallin splice junction mutation for autosomal dominant congenital cataract on chromosome 17q. Investigative Ophthalmology and Visual Science 41:3278-3285, 2000.
  37. Johannes, M., Geyer, D.D., Berry, R., Walter, N.A.R., Sikela, J.M. and Bateman,J.B.: Comparison of fluorescent genotyping methods. Analytical Biochemistry 262:193-195, 1998.
  38. Deloukas, P., Schuler, G.D., Gyapay, G., Beasley, E.M., Soderlund, C., RodriguezTome, P., Hui, L., Matise, T.C., McKusick, K.B., Beckmann, J.S., Bentolila, S., Bihoreau, M.T., Birren, B.B., Browne, J., Butler, A., Castle, A.B., Chiannikulchai, N., Clee, C., Day, P.J.R., Dehejia, A., Dibling, T., Drouot, N., Duprat, S., Fizames, C., Fox, S., Gelling. S., Green, L., Harison, P., Hocking, R., Holloway, E., Hunt S., Keil, S., Lijnzaad, P., LouisDitSully, C., Ma, J., Mendis, A., Miller, J., Morissette, J., Muselet, D., Nusbaum, H.C., Peck, A., Rozen, S., Simon, D., Slonim, D.K., Staples, R., Stein, L.D., Stewart, E.A., Suchard, M.A., Thangarajah, T., VegaCzarny, N., Webber, C., Wu, X., Auffray, C., Nomura, N., Sikela, J.M., Polymeropoulos, M.H., James, M.R., Lander, E.S., Hudson, T.J., Myers, R.M., Cox, D.R., Weissenbach, J., Boguski, M.S., and Bentley, D.R.: A physical map of 30,000 human genes. Science 282:744-746, 1998.
  39. Radcliffe, R.A., Walter, N.A.R., Sikela, J.M., and Erwin, V.G.: Discovery of a new Pomc-l allele in the LS x SS recombinant inbred strains: relationship to locomotor behavior. Behavior Genetics 28:4955, 1998.
  40. Sjoholt, G. Molven, A., Lovlie, R., Wilcox, A., Sikela, J.M. and Steen, V.M.: Genomic structure and chromosomal localization of a human myo-inositol monophosphatase gene (IMPA). Genomics 45:113-122, 1997.
  41. Brady KP, Rowe LB, Her H. Stevens TJ, Eppig J. Sussman DJ, Sikela J. Beier DR. Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism (SSCP) analysis. Genome Research 7:1085-1093, 1997.
  42. Wafford, K.A., Thompson, S.A., Thomas, D. Sikela, J., Wilcox, A.S. and Whiting, P.J.: Functional characterization of human GABAA receptors containing the alpha 4 subunit. Molecular Pharmacology 50:670-678, 1996.
  43. Schuler, G.D., Boguski, M.S., Stewart, E.A., Stein, L.D., Gyapay, G., Rice, K., White, R.E., RodriguezTome, P., Aggarwal, A., Bajorek, E., Bentolila, S., Birren, B.B., Butler, A., Castle, A.B., Chiannilkulchai, N., Chu, A., Clee, C., Cowles, S., Day, P.J.R., Dibling, T., Drouot, N., Dunham, I., Duprat, S., East, C., Edwards, C., Fan, J.B., Fang, N., Fizames, C., Garrett, C., Green, L., Hadley, D., Harris, M., Harrison, P., Brady, S., Hicks, A., Holloway, E., Hui, L., Hussain, S., LouisDitSully, C., Ma, J., MacGilvery, A., Mader, C., Maratukulam, A., Matise, T.C., McKusick, K.B., Morissette, J., Mungall, A., Muselet, D., Nusbaum, H.C., Page, D.C., Peck, A., Perkins, S., Piercy, M., Qin, F., Quackenbush, J., Ranby, S., Reif, T., Rozen, S., Sanders, C., She, X., Silva, J., Slonim, D.K., Soderlund, C., Sun, W.L., Tabar, P., Thangarajah, T., VegaCzarny, N., Vollrath, D., Voyticky, S., Wilmer, T., Wu, X., Adams, M.D., Auffray, C., Walter, N.A.R., Brandon, R., Dehejia, A., Goodfellow, P.N., Houlgatte, R., Hudson Jr., J.R., Ide, S.E., Iorio, K.R., Lee, W.Y., Seki, N., Nagase, T., Ishikawa, K., Nomura, N., Phillips, C., Polymeropoulos, M.H., Sandusky, M., Schmitt, K., Berry, R., Swanson, K., Torres, R., Venter, J.C., Sikela, J.M., Beckmann, J.S., Weissenbach, J., Meyers, R.M., Cox, D.R., James, M.R., Bentley, D., Deloukas, P., Lander, E.S., and Hudson, T.J.: A gene map of the human genome. Science 274:540-546, 1996.
  44. Nakatsu, Y., Tyndale, R.F., DeLorey, T.M., DurhamPierre, D., Gardner, J.M., McDaniel, H.J., Nguyen, Q., Wagstaff, J., Lalande, M., Sikela, J.M., Olsen, R.W., Tobin, A.J. and Brilliant, M.H.: A cluster of three GABAA receptor subunit genes is deleted in a neurological mutant of the mouse p locus. Nature 364:448450, 1996.
  45. Gordon, D.F., Wagner, J., Atkinson, B.L., Chiono, M., Berry, R., Sikela, J. and GutierrezHartmann, A.: Human Cartl: structural organization, chromosomal localization, and functional analysis of a cartilage specific homeodomain cDNA. DNA and Cell Biology15:531-538, 1996.
  46. Orlicky, D.J., Berry, R. and Sikela, J.M.: Human chromosome 1 localization of the gene for a prostaglandin F2 receptor negative regulatory protein. Human Genetics 97:655-658, 1996.
  47. Renouf, S., Beullens, M., Wera, S., Van Eynde, A., Sikela, J., Stalmans, W. and Bollen, M.: Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase1. FEBS Letters 375:75-78, 1995.
  48. Callen, D.F., Lane S.A., Kozman, H., Kremmidiotis, G., Whitmore, S.A., Lowenstein, M., Doggett, N.A., Kenmochi, N., Page, D.C., Maglott, D.R., Nierman, W.C., Murakawa, K., Berry, R., Sikela, J.M., Houlgate, R., Auffray, C. and Sutherland, G.R.: Integration of transcript and genetic maps of chromosome 16 at near 1Mb resolution: demonstration of a "hot spot" for recombination at 16p12. Genomics 29:503-511, 1995.
  49. Berry, R., Stevens, T.J., Walter, N.A.R., Wilcox, A.S., Rubano, T., Hopkins, J.A., Weber, J., Goold, R., Soares, M.B. and Sikela, J.M.: Genebased STSs as the basis for a human gene map. Nature Genetics 10:415-423, 1995.
  50. Berry, R., Walter, N.A.R., Stevens, T.J., Rubano, T., Wilcox, A.S., Hopkins, J.A. and Sikela, J.M.: A strategy for the identification of candidate genes for alcohol related phenotypes and other human disorders using rapid PCR mapping of genebased STSs. Alcoholism: Clinical and Experimental Research 19:821-823, 1995.
  51. Glod, J. and Sikela, J.M.: Ca2+/calmodulin dependent protein kinase IV. In Factsbook on Protein Kinases, S. Hanks and D.G. Hardie, editors, 1:137-139, 1995.
  52. Greger, V., Knoll, J.H.M., Woolf, E., Glatt, K., Tyndale, R.F., DeLorey, T.M., Olsen, R.W., Tobin, A., Sikela, J.M., Nakatsu, Y., Brilliant, M.H., Whiting, 3 subunit gene (GABRG3) is tightly P.J. and LaLande M.: The GABA receptor 5 subunit gene (GABRA5) on human chromosome 15q11q13 and is linked to the transcribed in the same orientation. Genomics 26:258264, 1995.
  53. Hellevuo, K., Berry, R., Sikela, J.M. and Tabakoff, B.: Localization of the gene for a novel human adenyl cyclase (ADCY7) to chromosome 16. Human Genetics 95:197-200,1995.
  54. Coon H, Hoff M, Holik J, Umar F, Robertson M, Reimherr F, Wender P, Vest K, Myles-Worsley M, Plaetke R, Gershon ES, DeLisi LE, Shields G, Dale P, Polloi A, Waldo M, Leonard S, Sikela J, Freedman R, Byerley W. A search for mutations in the b1 GABAA receptor in patients with schizophrenia. American Journal of Medical Genetics: Neuropsychiatric Genetics 54:12-20, 1994.
  55. Tokumitsu, H., Brickey, D.A., Glod, J., Sikela, J.M. and Soderling, T.R.: Activation mechanism for Ca2+/calmodulin dependent protein kinase IV. Identification of a brain CaM kinase IV kinase. J. Biol. Chem. 269:28640-28647, 1994.
  56. Le Bourdelles, B., Wafford, K.A., Kemp, J.A., Marshall, G., Bain, C., Wilcox, A.S., Sikela, J.M. and Whiting, P.J.: Cloning, functional coexpression and pharmacological characterization of cDNAs encoding the human NMDA receptor NR1 and NR2A subunits. J. Neurochemistry 62:2091-2098, 1994.
  57. Sikela, J.M., Stevens, T.J., Hopkins, J.A., Wilcox, A.S., Glod, J., Khan, A.S. and Orpana, A.K.: Abundance screening of cDNA libraries. In Automated DNA sequencing and analysis techniques, M.D. Adams, C. Fields & J.C. Venter, editors, 123-125, 1994.
  58. Hadingham, K.L., Wingrove, P.B., Wafford, K.A., Bain, C., Kemp, J.A., Palmer, K.J., Wilson, A.W., Wilcox, A.S., Sikela, J.M., Ragan, C.I. and Whiting, P.J.: The role of the beta subunit in determining the pharmacology of human GABAA receptors. Molecular Pharmacology 44:1211-1218, 1993.
  59. Byerley, W., Khan, A.S., Holik, J., Hoff, M. and Sikela, J.M.: Dinucleotide repeat polymorphism in the 3' untranslated region of an anonymous brain cDNA mapping to chromosome 2. Human Molecular Genetics 2:183-189, 1993.
  60. Gulya, K., Orpana, A.K., Sikela, J.M. and Hoffman, P.L.: Prodynorphin and vasopressin mRNA levels are differentially affected by chronic ethanol ingestion in the mouse. Molecular Brain Research 20:18,1993.
  61. Polymeropoulos, M.H., Xiao, H., Sikela, J.M., Adams, M., Venter, and Merril, C.R.: Chromosomal distribution of 320 genes from a brain cDNA library. Nature Genetics 4:381-386,1993.
  62. Sikela, J.M. and Auffray, C. Finding new genes faster than ever. Nature Genetics 3:189-191,1993.
  63. Knoll, J.H.M., Sinnett, D., Wagstaff, J., Glatt, K., Wilcox, A.S., Whiting, P., Wingrove, P., Sikela, J.M. and Lalande, M.: FISH ordering of reference markers and of the gene α5 subunit of the GABAA receptor within the Angelman and PraderWilli syndrome chromosomal regions. Human Molecular Genetics 2:183-189,1993.
  64. Drebing, C.A., Sikela, J.M., Hopkins, J.A., Byerley, W., Khan, A.S., Leonard, S. and Freedman, R.: Comparison of the DNA sequence of the protein coding region of beta nerve growth factor in schizophrenics and normals. Psychiatric Genetics 3:21-27,1993.
  65. Bowers, B.J., Parham, C.L., Sikela, J.M. and Wehner, J.M.: Isolation and isozyme. sequence of a mouse brain cDNA coding for protein kinase C-γ isozyme. Gene 123:263-265, 1993.
  66. Zahniser, N.R., Buck, K.J., Curella, P., McQuilkin, S., WilsonShaw, D., Miller, C.L., Klein, R.L., Heidenreich, K.A., Keir, W.J., Sikela, J.M. and Harris, R.A.: GABAA receptor function and regional analysis of subunit mRNAs in long-sleep and short-sleep mouse brain. Molecular Brain Research 14:196-206, 1992.
  67. Khan, A.S., Wilcox, A.S., Polymeropoulos, M.H., Hopkins, J.A., Stevens, T.J., Robinson, M.R., Orpana, A.K. and Sikela, J.M.: Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nature Genetics 2:180-185, 1992.
  68. Warrington, J.A., Bailey, S.K., Armstrong, E., Aprelikova, O., Alitalo, K., Saltman, D., Wilcox, A.S., Sikela, J.M., Wolf, S.F., Lovett, M. and Wasmuth J.J.: A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5. Genomics 13:803-808, 1992.
  69. Wilcox, A.S., Warrington, J.A., Gardiner, K., Berger, R., Whiting, P., Altherr, M.R., Wasmuth, J.J., Patterson, D. and Sikela, J.M.: Human chromosomal localization of genes encoding the α1 and γ 2subunits of the GABAA receptor indicates members of this gene family are often clustered in the genome. Proc. Natl.Acad.Sci. USA 82:5857-5861,1992.
  70. Buck, K.J., Harris, R.A. and Sikela, J.M.: A general method for quantitative PCR analysis of mRNA levels for members of gene families: application to the GABAA receptor. BioTechniques 11:636-641, 1991.
  71. Sikela, J.M., WilsonShaw, D., Khan, A.S., Lin, L.H., Leidenheimer, N., Gambarana, C. and Siegel, R.E.: Localization and functional expression of alternatively spliced forms of the γ2 subunit of the GABAA receptor. Molecular and Cellular Neuroscience 2:338-343, 1991.
  72. Buck, K.J., Hahner, L., Sikela, J.M. and Harris, R.A.: Chronic ethanol treatment alters brain levels of GABAA receptor subunit mRNAs: relationship to genetic differences in ethanol withdrawal seizure sensitivity. Journal of Neurochemistry 57:1452-1455, 1991.
  73. Jones, D.A., Glod, J., WilsonShaw, D., Hahn, W.E. and Sikela, J.M.: cDNA sequence and differential expression of the mouse Ca2+/calmodulin dependent protein kinase IV gene. FEBS Letters 289:105-109, 1991.
  74. Wafford, K.A., Burnett, D.M., Leidenheimer, N.J., Burt, D., Dunwiddie, T.V., Harris, R.A. and Sikela, J.M.: Ethanol sensitivity of the GABAA receptor expressed in Xenopus oocytes requires eight amino acids contained in the γ2L subunit. Neuron 7:27-33, 1991.
  75. WilsonShaw, D., Robinson, M., Gambarana, C., Siegel, R.E. and Sikela, J.M.: A novel subunit of the GABAA receptor identified using the polymerase chain reaction. FEBS Letters 284:211215, 1991.
  76. Wilcox, A.S., Khan, A.S., Hopkins, J.A. and Sikela, J.M.: Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome. Nucleic Acids Research 12:1837-1843, 1991.
  77. Khan, A.S., Wilcox, A.S., Hopkins, J.A. and Sikela, J.M.: Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers. Nucleic Acids Research 12:17-15,1991.
  78. Keir, W.J., Kozak, C.A., Deitrich, R.A. and Sikela, J.M.: The cDNA sequence and chromosomal location of the murine GABAA α1 receptor gene. Genomics 2:390-395,1991.
  79. Keir, W.J., Deitrich, R.A. and Sikela, J.M.: Sequence comparison of the GABAA receptor α1 subunit cDNA from long- and short-sleep mice by direct sequencing of PCR amplified cDNA. Annals of the New York Academy of Sciences 625:551-554, 1991.
  80. Sikela, J.M., Adamson, M.C., WilsonShaw, D. and Kozak, C.A.: Genetic mapping of the gene for Ca2+/calmodulin-dependent protein kinase IV (Camk-4) to mouse chromosome 18. Genomics 8:579-582, 1990.
  81. Sikela, J.M., Keir, W.J. and WilsonShaw, D.R.: Molecular genetic study of the role of the GABA/benzodiazepine receptor in neurosensitivity to alcohol. National Institute on Alcohol Abuse and Alcoholism Research Monograph: Initial Sensitivity to Alcohol 20:317-321, 1990.
  82. Guerini, D., Krinks, M.H., Sikela, J.M., Hahn, W.E., and Klee, C.B.: Isolation and sequence of a cDNA clone for human calcineurin B. the Ca2+ binding subunit of the Ca2+/calmodulin stimulated protein phosphatase. DNA 8:675-682, 1989.
  83. Owens, G.P., Sinha, A.K., Sikela, J.M. and Hahn, W.E.: Sequence and expression of the murine diazepam binding inhibitor. Molecular Brain Research 6:101-108, 1989.
  84. Sikela, J.M., Law, M.L., Kao, F.T., Hartz, J.H., Wei, Q. and Hahn, W.E.: Human chromosome assignment and regional localization of the gene encoding brain Ca2+/calmodulin dependent protein kinase Type IV. Genomics 4:21-27,1989.
  85. Sikela, J.M. and W.E. Hahn: Screening an expression library with a ligand probe: Isolation and sequence of a cDNA corresponding to a brain calmodulin binding protein. Proc. Natl. Acad. Sci. USA 84:3038-3042, 1987.
  86. Dush, M.K., J.A. Tischfield, S.A. Khan, E. Feliciano, J.M. Sikela, C.A. Kozak, P.J. Stambrook: An unusual adenine phosphoribosyl transferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs. Mol. Cell. Biol. 6:4161-4167, 1986.
  87. Hahn, W.E., N. Chaudhari, J.M. Sikela and G.P. Owens: Messenger RNA in the brain. In: Role of RNA and DNA in Brain Function, A. Giuditta, B. Kaplan, C. Zomzely-Neurath, eds. (Boston: Martinus Nijhoff Publishing), pp. 10-22, 1986.
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