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Department of Pharmacology

Department of Pharmacology
 

Electrophysiology


​The Electrophysiology Users group is made up of graduate students, PRA's, post-docs, and faculty that are using electrophysiology techniques to answer research questions.  The group meets monthly to discuss fundamental electrophysiology topics to enhance our knowledge of our specialty.  The meetings are fairly informal and foster open discussion and networking among members.  Electrophysiologists at all levels of expertise are welcome to attend.  Bring your knowledge and your questions!

 

Date
Topic
Moderator
03/27/2014
Solutions: ACSF, cutting solutions, additives
Heather
04/24/2014
Sacrifice methods: Anesthesia (isoflurane vs. ketamine), Cervical dislocation Decapitation, Temperature, and Speed
Slicing techniques and equipment: Tissue choppers, Vibratomes to Leica, Others?
Steve
05/22/2014
Noise reduction and hum chasing, Grounds and ground loops Ambient noise sources, Filtering noise
Jenni
06/26/2014
Patch configurations (cell-attached, whole cell, inside-out, perforated patch) and advantages of each
Pathcing onto a neuron or other cell
Stability of responses (which responses?)
Current and voltage clamp
I-V curves and Rectification
Series Resistance
Bill Sather
07/24/2014
Recording systems and filters    
Components of a hippocampal field potential (stimulus artifact,
fiber potential, epsp, population spike contamination
Population spikes, Laminar analysis (how signals vary across synaptic fields),
Signal components can detect different things (amplitude vs. slope),
Methods of slope measurement
Isolated field responses
I-O curves, Stimulus intensity vs. response (stimulus or amplitude)
Fiber potential size vs. response (stimulus or amplitude)
Ceilings and floors (why I-O curves are important)
Funny or strange signals and what they might mean
Paired-pulse responses and pre-vs. post-synaptic mechanisms
Recordings in other brain regions?
Access resistance
Field recordings: Electrode impedance and electrode solution (ACSF vs. NaCl or KCl)
Patch electrodes: pulling methods, impedance, solutions (Cs, Kgluconate, others)
Software programs available
Benke and Ron

08/28/2014

Drug application, Perfusion, Recycling, Flow-through, Syringe pumps, Rapid-switch stream perfusion

Phil

​09/25/2014

Stimulation techniques, Bipolar

Current vs. voltage simulation (which is better?)

Monopolar stimulation, Uncaging and focal stimulation

Benke

​10/23/2014

Data Analysis and Statistics
Rejection of “bad” data
T-test vs. ANOVA
One-sample T-test
Smirnov-? Test?
Other stats

TBD​

 

Name ​Email Address ​Building ​Room Number ​Research Interest
Kelsey Barcomb kelsey.barcomb@ucdenver.edu ​RC1-N ​6401G Field recordings from hippocampal slices measuring basal transmission and LTP during treatment with pharmacological agents. Slices prepared with vibratome.
Paul Bernard, PhD

paul.b.bernard@ucdenver.edu

​RC1-N ​6400A1 ​Field recordings from adult rat hippocampal slices, LTD, mGluR LTD, LTP, recirculation, and drug perfusion.  Beginning whole cell recording from adult rat hippocampal slices, voltage clamp, AMPA and NMDA receptor currents. 
​Tim Benke, MD, PhD

tim.benke@ucdenver.edu

​RC1-N ​6104 ​Whole cell patch-clamp and field recordings in hippocampal slices and cultures. Glutamate receptor pharmacology. Biophysical analysis and modeling of glutamate receptor-channel function.
​Heather Caballes, PhD

heather.caballes@ucdenver.edu

​RC1-N ​6400A2 ​Whole Cell, Voltage Clamp recording, Acute Slices from Rat Hippocampus, AMPA and NMDA receptors currents from CA1 pyramidal neurons. 
​Anna Castano

anna.castano@ucdenver.edu

​RC1-N ​6400A1 ​​Learning E-phys and field recordings in adult rat hippocampal slices. Learning LTP and LTD protocols with drug treatments.
​Christian Cea-Del Rio

christian.cea-delrio@ucdenver.edu

School of Pharmacy(V20)​

​3116 ​Whole cell, voltage and current clamp, stimulation recordings (long and short term synaptic plasticity), transversal hippocampal and coronal somatosensory cortex slices, inhibitory currents and interneuron cell type recordings, neuromodulatory responses (specifically cholinergic and endocannabinoid responses).
​Steve Coultrap, PhD steve.coultrap@ucdenver.edu

​RC1-N

​6401G ​I have a pretty extensive experience with extracellular recordings from hippocampal slices of both rats and mice of a variety of ages (including slices from 2 year old rats). I have also done a lot of biochemistry with slices. I have some experience with hippocampal slice cultures. In grad school I did a lot of 2-electrode voltage clamp recordings from Xenopus oocytes.
​Kevin Crosby, PhD                kevin.crosby@ucdenver.edu ​RC1-N ​6401K ​I am a complete novice when it comes to E-phys, but I am quite interested in learning as much as possible. I have experience with a range of (quantitative) fluorescence-based microscopy techniques and I feel a combination of these methods is a potentially powerful approach that I would like to apply to the study of AKAP79 mediated excitation-transcription response from L-VGCCs in hippocampal neurons.
​Philip Dittmer, PhD philip.dittmer@ucdenver.edu ​RC1-N ​7401k ​Whole Current Recording in Pirmary Neurons
​Jeff Forte jeffrey.forte@ucdenver.edu ​RC1-N ​6402A ​Whole cell, Voltage & Current Clamp, Dissociated Neuronal Cultures & Cultured Organotypic Hippocampi
​Sam Freedman samuel.freedman@ucdenver.edu ​School of Pharmacy (V20) ​3460D Whole cell patch clamp recordings, excitability testing with rat cultured neurons.​
​Ron Freund, PhD ​​ron.freund@ucdenver.edu ​RC1-N ​6400C ​Field recording on hippocampal slices prepared either by tissue chopper or Vibratome1000, Drug perfusion, recirc. or flow-thru LTD, LTP
​Dayton Goodell dayton.goodell@ucdenver.edu ​RC1-N ​6401G ​Field recordings from hippocampal slices investigating NMDA dependent LTD
​Molly M. Hunstman, PhD molly.hunstman@ucdenver.edu ​Schoo of Pharmacy (V20) ​3121 ​Inhibitory synaptic transmission, GABAA receptor-mediated currents in cortical and amygdala neurons, primary somatosensory cortex, Fragile X Syndrome and epilepsy, whole cell voltage clamp, acute brain slices, biocytin filling of physiological identified cells.
​Frances Meredith, PhD frances.meredith@ucdenver.edu ​RC2 ​7470E ​Whole cell, voltage and current clamp, acute slices of the crista ampullaris (vestibular sensory epithelium). Ionic currents, firing, EPSCs and EPSPs in the calyx afferent terminal.
​Ricardo H Pineda, PhD ricardo.pineda@ucdenver.edu ​RC1-N ​7401K ​Regulation of L-Type VGCC in hippocampal neurons, VG potassium channels, chronic pain, ALS, whole cell patch-clamp (voltage- and current-clamp) in vivo, acutely isolated and cultured cells, acute and organotypic brain and spinal cord slices.
​Nidia Quillinan, PhD

nidia.quillinan@ucdenver.edu

​I perform whole cell recording from Purkinje cells and CA1 neurons in acute brain slices from post-ischemic juvenile and adult mouse brain. I use whole cell recording to examine evoked, spontaneous and iontophoretic postsynaptic currents, and LTD at parallel fiber to Purkinje cell synapse. I also have experience with intracellular recording (sharps) in acute brain slices.
​Katie Rennie, PhD                katie.rennie@ucdenver.edu ​RC2 ​7025 ​Whole Cell Voltage Clamp and Current Clamp, Peripheral Vestibular System (Isolated cells, Cultures and Slices), Afferent and Efferent Signaling, K+ channels and glutamate receptors.

 

Resources

Hille, B (2001) Ion Channels of Excitable Membranes, Third Edition.  Sunderland, MA:  Sinauer Associates, Inc. 

Sakmann, B. and Neher, E. (1995) Single-Channel Recording, Second Edition. New York and London: Plenum Press.

Schurr, A. and Rigor, B.M. (1995) Brain Slices in Basic and Clinical Research. Boca Raton, London, and Tokyo: CRC Press.

Walz, W., Boulton, A.A., and Baker, G.B. (2002) Patch-Clamp Analysis Advanced Techniques. Totowa, New Jersey: Humana Press.

The Axon Guide:   http://www.culturacientifica.org/textosudc/Axon_Guide.pdf

Brain Slice Methods website:  http://www.brainslicemethods.com/