Allen Waziri, MD
Department of Neurosurgery
Room 5001, Academic Office Building One
12631 E. 17th Ave.
Aurora, CO 80045
(303) 724-2397 (clinical office)
(303) 724-4134 (lab office)
Our research efforts are focused on glioblastoma (GBM), the most malignant primary brain tumor and one of the deadliest cancers known to modern medicine. In our ongoing study of the suppression of anti-tumor immune responses in affected patients, we recently identified a new source of immunosuppression in GBM patients that is associated with increased numbers of circulating activated neutrophils. A major focus of the lab is developing further insight into the role that activated neutrophils play in the biology of GBM and the benefits that may be derived by targeting this population.
A second area of interest involves tumor cell invasion in GBM, which is a critical source of treatment failure in affected patients. We have developed a model whereby patient tumor samples are kept alive in culture for several weeks, allowing us to evaluate the behavior of tumor cells within the “ex vivo” microenvironment. Labeling of tumor cells and time-lapse laser confocal microscopy are used to “track” tumor cells as they migrate over time, which permits comparative analysis of factors associated with invasive characteristics as well as “personalized” therapeutic targeting of tumor cell movement on a patient-specific basis.
Trisha Sippel (CANB Program)
Jonathon Parker (MSTP/CANB Program)
Selected recent publications:
Waziri A. Glioblastoma-derived mechanisms of systemic immunosuppression. Neurosurg Clin N Amer. 2010 Jan; 21(1): 31-42.
Sippel T, White J, Nag K, Tsvankin V, Klaassen M, Kleinschmidt-DeMasters BK, Waziri A. Neutrophil degranulation and immunosuppression in patients with GBM: restoration of cellular immune function by targeting Arginase I. Clin Cancer Res. 2011 Nov 15; 17(22): 6992-7002.
Parker JJ, Dionne KR, Massarwa R, Klaassen M, Foreman NK, Niswander L, Canoll P, Kleinschmidt-DeMasters BK, Waziri A. Gefitinib selectively targets tumor cell migration in EGFR-amplified human glioblastoma (submitted).
Favorable Prognosis in Patients with High-Grade Glioma with Radiation Necrosis: The University of Colorado Reoperation Series.
Rusthoven KE, Olsen C, Franklin W, Kleinschmidt-Demasters BK, Kavanagh BD, Gaspar LE, Lillehei K, Waziri A, Damek DM, Chen C.
Int J Radiat Oncol Biol Phys. 2010 Aug 21. [Epub ahead of print]PMID: 20732762 [PubMed - as supplied by publisher]
Intracortical EEG for the Detection of Vasospasm in Patients with Poor-Grade Subarachnoid Hemorrhage.
Stuart RM, Waziri A, Weintraub D, Schmidt MJ, Fernandez L, Helbok R, Kurtz P, Lee K, Badjatia N, Emerson R, Mayer SA, Connolly ES, Hirsch LJ, Claassen J.
Neurocrit Care. 2010 Jul 22. [Epub ahead of print]PMID: 20652443 [PubMed - as supplied by publisher]
Intracranial multimodal monitoring for acute brain injury: a single institution review of current practices.
Stuart RM, Schmidt M, Kurtz P, Waziri A, Helbok R, Mayer SA, Lee K, Badjatia N, Hirsch LJ, Connolly ES, Claassen J.
Neurocrit Care. 2010 Apr;12(2):188-98.PMID: 20107926 [PubMed - indexed for MEDLINE]
Glioblastoma-derived mechanisms of systemic immunosuppression.
Neurosurg Clin N Am. 2010 Jan;21(1):31-42. Review.PMID: 19944964 [PubMed - indexed for MEDLINE]
Immune gene and cell enrichment is associated with a good prognosis in ependymoma.
Donson AM, Birks DK, Barton VN, Wei Q, Kleinschmidt-Demasters BK, Handler MH, Waziri AE, Wang M, Foreman NK.
J Immunol. 2009 Dec 1;183(11):7428-40. Epub 2009 Nov 16.PMID: 19917695 [PubMed - indexed for MEDLINE]
Intracortical electroencephalography in acute brain injury.
Waziri A, Claassen J, Stuart RM, Arif H, Schmidt JM, Mayer SA, Badjatia N, Kull LL, Connolly ES, Emerson RG, Hirsch LJ.
Ann Neurol. 2009 Sep;66(3):366-77.PMID: 19798724 [PubMed - indexed for MEDLINE]
Rapid recurrence and malignant transformation of pilocytic astrocytoma in adult patients.
Ellis JA, Waziri A, Balmaceda C, Canoll P, Bruce JN, Sisti MB.
J Neurooncol. 2009 Dec;95(3):377-82. Epub 2009 Jun 17.PMID: 19533024 [PubMed - indexed for MEDLINE]
The survival impact of postoperative infection in patients with glioblastoma multiforme.
Bohman LE, Gallardo J, Hankinson TC, Waziri AE, Mandigo CE, McKhann GM 2nd, Sisti MB, Canoll P, Bruce JN.
Neurosurgery. 2009 May;64(5):828-34; discussion 834-5.PMID: 19404146 [PubMed - indexed for MEDLINE]
Initial surgical experience with a dense cortical microarray in epileptic patients undergoing craniotomy for subdural electrode implantation.
Waziri A, Schevon CA, Cappell J, Emerson RG, McKhann GM 2nd, Goodman RR.
Neurosurgery. 2009 Mar;64(3):540-5; discussion 545.PMID: 19240617 [PubMed - indexed for MEDLINE]
Dynamics of central and peripheral immunomodulation in a murine glioma model.
Kennedy BC, Maier LM, D'Amico R, Mandigo CE, Fontana EJ, Waziri A, Assanah MC, Canoll P, Anderson RC, Anderson DE, Bruce JN.
BMC Immunol. 2009 Feb 18;10:11.PMID: 19226468 [PubMed - indexed for MEDLINE]