The UK suffers from high levels of wildlife-associated spread of bovine tuberculosis. Cattle movement, exportation and professions based upon animal husbandry have suffered. My research was validation of a model of bovine tuberculosis that demonstrated lesion-level pathology, demonstrated consistent repeatability, and aided in providing correlates of reduced disease traits employed for vaccine studies. As a research team member, I demonstrated that in vitro data scoring correlated with in vivo lesions, and greater lesion-level pathology characterization aided comprehension of temporal and spatial disease advancement. My role was to collect tissues and provide gross and microscopic interpretation.
- Johnson, L.K., Gough, J., Spencer, Y., Hewinson, G., Vordermeier, M., Wangoo, A. Immunohistochemical markers augment evaluation of vaccine efficacy and disease severity in bacillus Calmette-Guerin (BCG) vaccinated cattle challenged with Mycobacterium bovis. Veterinary Immunology and Immunopathology. 111: 219-229, 2006 PMID:16540176
- Liebana, E., Marsh, S., Gough, J., Nunez, A., Vordermeier, M., Whelan, A., Spencer, Y., Clifton-Hadley, R., Hewinson, G., Johnson, L. Distribution and activation of T-lymphocyte subsets in tuberculous bovine lymph node granulomas. Veterinary Pathology, 44:366-372, 2007 PMID:17491079
- Liebana E., Johnson L.K., Gough J., Durr P., Jahans K., Clifton-Hadley R., Spencer Y., Hewinson R., Downs S. Pathology of naturally occurring bovine tuberculosis in England and Wales. The Veterinary Journal, 176:354-360, 2008 PMID:17728162
Documentation of the extent and severity of T lymphocyte infiltration into the brain in acute GVHD of mice. This research demonstrated the presence and extent of T lymphocytes within the brain, especially within the cerebellum. My role was to perform the gross necropsy, evaluate H&E stained slides and provide IHC.
- Hartrampf, S., Dudakov, J.A., Johnson, L.K., Smith, O.M., Tsai, J., Singer, N.V., West, M.L., Hanash, A., Albert, M.H., Liu, B., Toth, M., van den Brink, M.R. The central nervous system is a target of acute graft versus host disease in mice. Blood. 121(10):1906-10, 2013. PMID:23299314
- Hartrampf, S., Dudakov, J.A., Johnson, L.K., Smith, O.M., Tsai, J., Singer, N.V., West, M.L., Hanash, A., Albert, M.H., Liu, B., Toth, M., van den Brink, M.R. The CNS in acute GVHD: Development and effects. Biol Blood Bone Marrow Transplant (18)S359, 2012 PMID:23299314
MyD88 deficient mice are frequently models of immune-mediated disease. Reports of altered gastrointestinal mucus, and the impact of deficient innate immunity have contributed to greater understanding of the transfer of intestinal gram-negative bacteria to extra-intestinal locations. My role in this initial diagnostic case, which then highlighted the value of full necropsy for all unanticipated mortalities, was to collect tissues, culture and gram stain exudate and interpret lesions. The hyperplasia and hypertrophy of mesothelial cells was a previously unreported finding and highlighted the need for bacterial genetic characterization.
L.K., Johnson, A. Widi, S., Rowarth, A. Baxter. Abdominal distension and E. coli peritonitis in MyD88 deficient mice. Comp Med.; 65 (2):123-126, 2015 PMID:25926397
Partial listing of publications is available through this link: http://www.ncbi.nlm.nih.gov/myncbi/collections/bibliography/48669339/