Mg2+-homeostasis regulation in the immune system and the channel-kinase TRPM7
As opposed to Ca2+ the “signaling ion”, Mg2+ has commonly been described in literature as a “maintenance ion”. Despite the abundance and ubiquitous use of Mg2+
in all forms of life, and the well documented, crucial role of this
cation in cellular physiology, extremely little is known about molecular
components and mechanisms acting to regulate Mg2+-homeostasis, especially in vertebrates. Although there is still controversy whether cytosolic Mg2+
functions as a second messenger, over the last two decades, a growing
number of studies in various cellular systems could demonstrate that Mg2+
homeostasis is a very dynamic process, with multiple and complex forms
of regulation that probably plays an underestimated role in managing
cell function and metabolism. It appears that TRPM7 is an essential Mg2+ uptake and sensing pathway, and therefore a key-component of these cellular processes.
TRPM7 (as well as its closest relative TRPM6) is
a chanzyme, since it is the unique fusion of an ion channel with a
kinase domain. We have shown that TRPM7 is a Ca2+/Mg2+ permeable channel whose activity is modulated by intracellular Mg2+
and Mg-nucleotides. Furthermore, the lethal phenotype caused by the
inducible homozygous deletion of TRPM7 in the DT40 chicken B-lymphocytes
can be totally reverted by supplementing the growth medium with mM
concentrations of Mg2+. Amazingly, this phenotype is
extremely similar to that of patients suffering from an inherited form
of Hypomagnesemia who have been shown to bear mutations in TRPM6, the
closest relative of TRPM7.
Ongoing projects in my lab aim to better understand the signaling network(s) underlying cellular Mg2+-homeostasis
regulation, with a strong focus on the unique structural feature of
TRPM7 and its kinase domain. We have constructed a series of cell lines
stably and inducibly overexpressing TRPM7 kinase mutants, as well as
isolated cytoplasmic TRPM7 domains. We are studying the effects of these
mutations on channel function as well as on cellular physiology in the
TRPM7-deficient DT40 cells. Our working hypothesis is that the TRPM7
channel and kinase activities are functionally linked, with the kinase
modulating channel function, and the channel influencing kinase
activity. In turn, the kinase might coordinate intracellular signaling
pathways with the Mg2+-status of the cell. We are therefore also interested in further identifying and characterizing substrates of TRPM7-kinase (funded by NIH grant R01GM90123).
Furthermore, we are investigating the effect of TRPM7-deficiency and Mg2+-homeostasis deregulation on B-cell receptor signaling (funded by NIH grant R21AI088421).