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DEPARTMENT OF IMMUNOLOGY & MICROBIOLOGY, A Leader in Immunology, Microbiology and Microbial Pathogenesis Research and Training

Immunology and Microbiology
 

Carsten Schmitz, M.D.

Associate Professor of Immunology & Microbiology


12800 E. 19th Ave., RC1-N 8106
Mail Stop 8333, Aurora, CO 80045

Phone: 303-270-2732
E-mail: carsten.schmitz@ucdenver.edu

The channel-kinases TRPM6/7 and their role in Mg2+-homeostasis regulation and nutritional signaling

As opposed to Ca2+ the “signaling ion”, Mg2+ has commonly been described in literature as a “maintenance ion”. Despite the abundance and ubiquitous use of Mg2+ in all forms of life, and the well documented, crucial role of this cation in cellular physiology, extremely little is known about molecular components and mechanisms acting to regulate Mg2+-homeostasis, especially in vertebrates. Although there is still controversy whether cytosolic Mg2+ functions as a second messenger, over the last two decades, a growing number of studies in various cellular systems could demonstrate that Mg2+ homeostasis is a very dynamic process, with multiple and complex forms of regulation that probably plays an underestimated role in managing cell function and metabolism. It appears that TRPM7 is an essential Mg2+ uptake and sensing pathway, and therefore a keycomponent of these cellular processes.

More recent epidemiological and genetic studies have shown a direct role of a TRPM7 T1482I genetic polymorphism in neurodegenerative disorders (gALS, gPD) and colorectal neoplasia, whereas mutations of the Mg2+ transporter MagT1 have been linked to XMEN (X-linked immunodeficiency with Magnesium defect, chronic Epstein–Barr virus infections and Neoplasia) in humans.

TRPM7 (as well as its closest relative TRPM6) is a chanzyme, since it is the unique fusion of an ion channel with a kinase domain (fig. 1). We have shown that TRPM7 is a Ca2+/Mg2+ permeable channel whose activity is modulated by intracellular Mg2+ and Mg-nucleotides. Furthermore, the lethal phenotype caused by the inducible homozygous deletion of TRPM7 in the DT40 chicken B-lymphocytes can be totally reverted by supplementing the growth medium with mM concentrations of Mg2+. Amazingly, this phenotype is extremely similar to that of patients suffering from an inherited form of Hypomagnesemia who have been shown to bear mutations in TRPM6, the closest relative of TRPM7.


Ongoing projects in my lab aim to better understand the signaling network(s) underlying cellular Mg2+-homeostasis regulation, with a strong focus on the unique structural feature of TRPM7 and its kinase domain. We have constructed a series of cell lines stably and inducibly overexpressing TRPM7 kinase mutants, as well as isolated cytoplasmic TRPM7 domains. We are studying the effects of these mutations on channel function as well as on cellular physiology in the TRPM7-deficient DT40 cells. Our working hypothesis is that the TRPM7 channel and kinase activities are functionally linked, with the kinase modulating channel function, and the channel influencing kinase activity. In turn, the kinase might coordinate intracellular signaling pathways with the Mg2+-status of the cell. We are therefore also interested in further identifying and characterizing substrates of TRPM7-kinase (funded by NIH grant R01GM90123). Furthermore, we are investigating the effect of TRPM7-deficiency and Mg2+-homeostasis deregulation on B-cell receptor signaling.