The Charles C. Gates Center for Regenerative Medicine and Stem Cell Biology, with support from the Dean of the School of Medicine, has established a Core facility that generates induced pluripotent stem cells (iPSCs) for the University of Colorado Anschutz Medical Center and other Colorado Universities.
Background: iPSCs have biological properties similar to embryonic stem (ES) cells. These cells can be generated from adult tissue cells (e.g. skin fibroblasts) by the introduction of a combination of transcription factors (reprogramming factors). iPSCs can be differentiated into many cell lineages. Consequently, it is anticipated that iPSCs will be essential tools for the generation of replacement tissues for regenerative medicine, for the screening of new drugs and small molecules aimed at modifying specific cellular processes, and for the design of new therapeutic strategies to treat genetic diseases. Further, the recent development of new genome editing tools, such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALENs), allow for the genetic modification of iPSCs, raising the possibility to generate gene-corrected iPSCs from patients with genetic diseases, which can be used to generate healthy tissues.
Services: The new iPSC Core generates custom-designed iPSCs from mouse and human cells, including disease-specific human iPSCs. iPSCs from other species are currently under development. The Core is currently using both lentiviral- and sendai viral vector systems to deliver reprogramming factors to cells. Both systems are efficient, with the latter system having the advantage to generate iPSCs with a non-DNA-integrating vector system.
In order to obtain further information about our services, service pricing, to schedule consultations or to placing service requests, please contact iPSCore@ucdenver.edu.
Peter J. Koch, Ph.D.
Core Director, Professor
"All communications with the Core must be routed through the iPSCore@ucdenver.edu mailbox. Briefly state your questions or concerns. Your email will be routed to appropriate core personnel."