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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center
 

Structural Biology Shared Resource


The Structural Biology Shared Resource (SBSR) provides University of Colorado Cancer Center members access to instrumentation and expertise in the use of X-ray Crystallography and Nuclear Magnetic Resonance Spectroscopy (NMR). It is one of only two NCI-designated cancer centers with X-ray Crystallography, NMR, high field NMR and synchrotron. 

The SBSR promotes the application of structural studies to the molecular basis of cellular processes that regulate the development and progression of cancer and application of that information to aid in drug design, complementing the strengths of the existing research programs in cell signaling, pharmacology, and cancer.

Services:

 
Structure Determination of Large Macromolecules (Proteins/Nucleic Acids)
Crystallization Screening
Preliminary Crystal Screening and Data Collection
Solution Structural Analysis of Small Molecules
Molecular Visualization
Experimental Design and Training
Solution Screening for Molecular Interactions

Equipment:


X-ray Instrumentation
  • The home source is composed of a Rigaku Micromax 007 high frequency microfocus X-ray generator, a VariMax high flux optic with adjustable divergence, an AFC11 4-axis goniometer, a Pilatus 200K 2D area detector, and an Oxford Cobra cryo-system. HKL-3000R is used for data collection and processing and is run on a Linux CentOS Dell Precision Workstation.
  • Rigaku/MSC Crystallization Robotics include: Alchemist liquid handling robot for optimization screen production, Phoenix drop setting robot, and 2 Desktop Minstrel imaging units and a plate hotel that are all networked and integrated with Crystal trak software.

    Instrumentation Available

    Spectrometers at CU Anschutz Medical Campus

    • Varian INOVA 500 MHz
      • 5 mm triple resonance 1H/13C/15N probe - sensitivity of 850:1 (ETB)
    • Varian INOVA 600 MHz
      • 5mm triple resonance  1H/13C/15N cold probe - sensitivity > 4000:1 (ETB)
    • Varian 900 MHz Direct Drive NMR system
      • 5 mm triple resonance 1H/13C/15N cold probe with Z-axis gradient
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