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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center
Tissue Culture Shared Resource at CU Cancer Center
 

Protein Production/MoAB/Tissue Culture


The Protein Production, Monoclonal Antibody, Tissue Culture Shared Resource (PPSR) supports
basic and translational research at CU Cancer Center Members with validated cell lines, preparation of hybridomas, production of monoclonal antibodies, isolation and titering of baculovirus expressing recombinant proteins, and large scale preparation of recombinant proteins and monoclonal antibodies.

PPSR provides centralized services to produce cancer-related proteins for CU Cancer Center members to study their mechanism of action in identified drugs and cancer-related biomarkers.

Services:

Custom Hybridoma Services/Monoclonal Antibody Production 

Recombinant Protein Expression (Baculovirus System)

Cell Line Repository/General Cell Culture Services

    • Seeds cultures/frozen ampules
    • >200 authenticated cancer cell lines, including 75 different lung cancer cell lines and 26 breast cancer cell lines
    • Large scale cell culture production
    • List of human cell lines available

Hyclone On-Site FBS/Media Supply Center

  • Hyclone characterized FBS tested/reserved Lot-freezer located in rm5404B from 8-3, distributed by shared resource staff.
  • Hyclone USDA FBS, Heat Inactivated FBS, Charcoal treated FBS, Calf and Horse serum
  • Hyclone Prices/order form
  • Hyclone Cell Cultrue Media and Supplements located in the hall RC1-S 5th floor hall.