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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center


Single and dual color fluorescence in situ hybridization (FISH) assays

  • Differentiation of human and rodent DNA by dual-color FISH assay in cell suspensions of formalin-fixed, paraffin-embedded cell lines.
  • Identification of specific aneuploidies in metaphase spreads and interphase nuclei, in  preparations from diffuse or solid tissues using satellite and locus-specific DNA probes.
  • Identification of particular human chromosomes or chromosome fragments in complex rearrangements and/or marker chromosomes using whole chromosome-specific painting probes (WCP) or a region-specific probe (Partial Chromosome Probes). WCPs are used also for identification of human chromosomes in hybrid cells.
  • DNA clone mapping (P1, PAC and BAC) on metaphase chromosomes of human, rat and mouse.
  • Investigation of genomic deletion, duplication or amplification in metaphase and in interphase nuclei in fresh or preserved biological preparations using laboratory developed FISH probes.
  • Detection of chromosome rearrangements in metaphase or interphase cells using probes encompassing or closely associated with the breakpoints.
  • Multicolor-FISH assay

  • Simultaneous hybridization of several probes to extended chromatin to determine the orientation and overlap of the tested sequences.
  • Analysis of multiple probes in a given cell for more accurate definition of its genomic status.  
  • Multicolor Karyotyping using spectral karyotyping in human, mouse and rat specimens (SKY) and multiplex-FISH (M-FISH) in human specimens.
  • Unbanded chromosome analysis

  • Breakage evaluation.
  • Cell proliferation index (mitotic index).
  • Ploidy evaluation
  • Giemsa-trypsin (GTG) banded chromosome analysis

  • Karyotype characterization of human and rodent specimens or cell lines in metaphase or prometaphase cells.
  • Determination of cell line homogeneity and evolution using metaphase analysis.
  • Identification of parental species in hybrid cell lines.
  • Additional procedures

  • Tissue culture - Initiation, maintenance, harvest, and freezing of cell cultures for cytogenetic purposes.
  • Development of FISH probes for enumeration, break-apart and fusion strategies
  • DNA labeling by nick-translation for FISH assays
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