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University of Colorado Cancer Center

University of Colorado Cancer Center, A National Cancer Institute-designated Comprehensive Cancer Center

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Flow Users Group

The Cancer Center Flow Cytometry Shared Resource sponsors the Front Range Flow Cytometry Users Group quarterly. Contact the FCSR to receive email announcements about the presentations.

 

Flow Cytometry Protocols

Krishan Stain for DNA Cell Cycle Analysis

  1. Prepare a single cell suspension with 0.1 to 1 x 10-6 cells per tube
  2. Wash cells with PBS, then centrifuge to pellet them
  3. Remove the supernatant PBS
  4. Vortex to dislodge the pellet
  5. Add 1 ml of Krishan’s stain (located in right column)
  6. Vortex again
  7. Refrigerate and let stand at least overnight before analysis

Luminex Multiplex Assays