Epithelial Tissue-Specific Stem Cells, Epithelial Repair
National Jewish Health, Main Health Campus
Must have completed 1 year of college and have experience
with mouse dissection.
Number of openings:1
This project will determine if tissue-specific stem cell
proliferation is regulated by the tumor-suppressor protein Nkx2.8. The studies
will utilize Nkx2.8 wild type and knockout mice and in vivo, in vitro, and
The student will learn the following experimental methods:
1) histological analysis of proliferation and differentiation in lung and
tracheal tissue sections from normal and naphthalene-injured mice; and 2) purification and in vitro analysis of
tissue-specific stem cell number, phenotype, and function. Human health relevance will be examined by
transplantation of wild type and knockout tissue-specific stem cells to the
skin or into the lungs. Transplanted cells will be evaluated for proliferation,
differentiation, and tumor-formation.