Signal pathways mediating autocrine growth of cancer cells
Lung Cancer, Head and Neck Cancer
University of Colorado Anschutz Medical Campus, Department of Craniofacial Biology
Must have completed one year of college
To use molecular biology and pharmacological inhibitors to identify specific growth factors, their receptor tyrosine kinases and signaling enzymes mediating autocrine growth of lung cancer and head and neck cancer cell lines.
Students will employ the following laboratory procedures:
- cell culture of human lung cancer and/or head and neck cancer cell lines;
- transfection experiments with expression vectors to over-express or silence specific gene products such as growth factors and growth factor receptors;
- perform assays to assess the effect of molecular perturbations or inhibitors on MAP kinase signaling, cell proliferation and anchorage-independent growth.