Signal pathways mediating autocrine growth of cancer cells and resistance to targeted therapeutics
Lung Cancer, Head and Neck Cancer, Mesothelioma
University of Colorado Anschutz Medical Campus, Department of Craniofacial Biology
Must have completed one year of college
To use molecular biology and pharmacological inhibitors to identify specific growth factors, receptor tyrosine kinases and signaling enzymes mediating autocrine growth of lung cancer cells as well as mechanisms of resistance to targeted therapeutics.
Students will employ the following laboratory procedures:
- cell culture of human lung cancer and/or head and neck cancer and/or mesothelioma cell lines;
- transfection experiments with expression vectors to over-express or silence specific gene products such as growth factors and growth factor receptors;
- perform assays to assess the effect of molecular perturbations or inhibitors on receptor tyrosine kinas activity, MAP kinase and AKT signaling, cell proliferation, anchorage-independent growth, and apoptosis.