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iPSC Culture Course at UC-AMC


​The iPSC Core at the Gates Center for Regenerative Medicine (www.medschool.ucdenver.edu/iPS ) is offering an iPS cell (iPSC) culture course. This one week course will focus on techniques required to maintain, preserve and amplify iPSC. Topics include: passage of mouse and human iPS cells; techniques to maintain iPSC in a pluripotent state; cryo-preservation and thawing of iPSC and transitioning of iPSC from feeders to feeder-free cell culture systems.  Proficient tissue cell culture skills are required for this hands-on class. The initial class will be restricted to 4 participants. Further classes will be offered depending on demand. To register, email gates.bioengineering@ucdenver.edu.  The cost is $1500 per participant.

Course Title:    iPS Cell (iPSC) Culture Course
When: Monday to Friday
9:00 am to 12:00 pm each day
Where: University of Colorado Anschutz Medical Campus
Research Complex 1 North
Focus: Techniques required to maintain, preserve and amplify iPSC
*Topics: Passage of mouse and human iPS cells
Techniques to maintain iPSC in a pluripotent state
Cryo-preservation and thawing of iPSC
Transitioning of iPSC from feeders to feeder-free cell culture systems
 
Curriculum  

iPSC Course Outline

  • Preparation of cell culture dishes I (e.g. matrigel preparation, coating and storage)
  • Preparation of cell culture dishes II (mitomycin C inactivation and plating of MEF cells)
  • iPS media preparation (mTeSR, TeSR 8 and DMEM/F12)
  • Culture and passage of mouse iPS on ME
  • Culture and passage of human iPS cells on matrigel and MEF 
  • Identifying differentiated and undifferentiated human iPS colonies  
  • Methods of removal differentiated colonies from iPS cultures  
  • Passaging, thawing and cryo-preservation of mouse and human iPS cells 
  • Transitioning of human iPS cells on MEF to feeder free system
Cost: ​ $1,500
​Registration: ​For further details and complete application package email gates.engineering@ucdenver.edu.
 
* Proficient tissue cell culture skills are required for this hands-on class
 
                             [Further classes will be offered depending on demand]