Please use the iPS Cell Service Request Form to initiate iPSC projects.
- Isolation, amplification and cryo-preservation of of primary fibroblast from human or mouse skin biopsy (for other cell types, please contact the Core)
- Reprogramming of mouse and human primary cells into iPS cells (cells from other species can be utilized as well, please inquire)
- The iPS Core utilizes both lentiviral and sendai viral transduction methods which can be used to routinely reprogram mouse embryonic fibroblasts (MEFs) or human primary fibroblast to a stem cell like state (iPSC) by over-expression of the four transcription factors Oct 4, Sox 2, Klf 4 and c-Myc (Yamanaka, 2006)
Validation and Characterization Services:
- Embryoid body formation
- Immunocytochemistry staining for pluripotency markers (Oct 4, Nanog, TRA-160, SSEA3, SSEA40)
- Mycoplasma testing
"All communications with the Core must be routed through the iPSCore@ucdenver.edu mailbox. Briefly state your questions or concerns. Your email will be routed to appropriate core personnel."
The cells produced by the Core are for non-profit academic research only. In order to obtain approval for the isolation of animal or human cell samples, please contact the UC Denver IACUC and COMIRB, respectively.