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iPSC Core


Please use the iPS Cell Service Request Form to initiate iPSC projects.


    • Isolation, amplification and cryo-preservation of of primary fibroblast from human or mouse skin biopsy (for other cell types, please contact the Core)
    • Reprogramming of mouse and human primary cells into iPS cells (cells from other species can be utilized as well, please inquire)
    • The iPS Core utilizes both lentiviral and sendai viral transduction methods which can be used to routinely reprogram mouse embryonic fibroblasts (MEFs) or human primary fibroblast to a stem cell like state (iPSC) by over-expression of the four transcription factors Oct 4, Sox 2, Klf 4 and c-Myc (Yamanaka, 2006)

Validation and Characterization Services:

    • Embryoid body formation
    • Immunocytochemistry staining for pluripotency markers (Oct 4, Nanog, TRA-160, SSEA3, SSEA40)
    • Karyotyping
    • Mycoplasma testing

"All communications with the Core must be routed through the mailbox. Briefly state your questions or concerns. Your email will be routed to appropriate core personnel."

The cells produced by the Core are for non-profit academic research only. In order to obtain approval for the isolation of animal or human cell samples, please contact the UC Denver IACUC and COMIRB, respectively.