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Molecular Biology Service Center

Services Overview

Automated DNA Sequencing 

  • Dye-terminator sequencing on ABI 3130x1 Genetic Analyzer capillary instrument
  • 800-1000bp Q20 read length
  • Online sample submission and results retrieval
  • Drop Box Locations at RC1-N, RC1-S, RC2
  • 24-36 hour turnaround time - Next business day results for samples submitted before 11AM
  • For plasmid sequencing, 20ul DNA template at 20-40ng/ul are required for EACH reaction
  • See Sample Submission Guidelines for details and requirements
  • List of Primers available provided by the MBCF
Automated Fragment Analysis 
  • ​Human Identification STR Analysis (Zygosity testing, human cell line authentication1)​

  • ​​​​Coming Soon! STR Testing for mouse- please stay tuned or inquire

  • Microsatellite analysis (Linkage analysis, mouse strain differentiation)

  • SNP detection
  • Determination of STR or VNTR number
  • MLPA analysis

Quantitative Real-Time PCR 

  • Roche LC480II
  • Gene Quantification and Expression analysis
  • High Resolution Melt analysis for Genotyping and Mutation detection
  • SNP Genotyping analysis (end-point genotyping, HRM)
  • Copy number analysis
  • Methylation Analysis
  • Consultation and training available for experimental design, reference gene selection, instrumentation use and results analysis

Mycoplasma Testing 

  • PCR based detection of various mycoplasma species
  • 50-100ng of genomic DNA for Sample Submission
  • We utilize the e-Myco PLUS PCR Mycoplasma Detection Kit
  • For Mycoplasma testing, 15ul gDNA template at >30ng/ul are required
Interspecies and Cross Contamination Testing 

  • Multiplex PCR assay that will screen for different species contamination in your cell lines
  • Will detect for the presence of human, mouse, rat, rabbit, and Chinese hamster
  • gDNA sample submitted for STR testing or mycoplasma testing can be used
  • 15ul gDNA template at >30ng/ul are required

Basic Molecular Biology Services  

  • Nucleic acid purification
  • Plasmid DNA preparation (small-large scale)
  • Restriction digestion and analysis
  • RT-PCR
  • PCR and PCR product purification

Custom Services

  • We perform a variety of protocol development and optimization projects for PCR, quantitative real-time PCR, Next Generation Sequencing (NGS), and various genotyping methods. Please inquire to see how we can assist you!
  • Please contact BDCBioResourcesCore​ by email or Mark Farrell​​ (Core Manager) for inquiries regarding your project.

Please do not hesitate to inquire about all services, details, specifics, pricing and sample submissions!

Barbara Davis Center
University of Colorado, Anschutz Medical Campus
1775 Aurora Court, 4th floor, Room 4207
Phone: 303-724-6825

Molecular Biology contact personnel:
Mark Farrell​Core Manager

  1. 'Notice Regarding Authentication of Cultured Cell Lines' from NIH.

The purpose of this website is to provide some basic information about the BDC Service Cores including contact information and links to on-line services where they are available. If you have questions about the BDC Service Cores please contact us (email or phone: 303-724-6836).