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University of Colorado Denver

Colorado School of Public Health
 

Biological Specimens


The following table lists the current biological specimen inventory available to researchers.
Collection Type ​Aliquots         
Available/Participants
CBD/PCBD 
Availabl​e
BeS   
Available​
Control
Available​
BAL cells ​184/ 85 ​42/23 ​142/62 ​0
BALF ​415/83 ​120/24 ​295/59 ​0
Blood-DNA ​9751/511 ​2721/151 ​3332/183 ​3698/177
PBMC 2659/487 ​788/145 ​1014/177 ​857/101
​Plasma ​3466/502 ​1029/147 ​1274/182 ​1163/172
 
Note: Transbronchial biopsy tissue is also available from 216 participants (3 slides each)
 
Storage Specifications:
 
Plasma 
Plasma samples are split into cryovials – 0.5 ml per vial. They are stored at -80°C in 2 inch fiberboard boxes.
 
Isolated DNA
DNA has been isolated from the blood and separated into aliquots at a volume of 0.1ml. Please note that the concentration of DNA (µg/ml) per aliquot is variable and requests will be made per volume, not concentration. DNA vials are labeled with printed labels and stored at -80 °C.
 
Cryopreserved cells  
Cells are split into 5 million cells per aliquot. They are stored in cryogenic safe plastic boxes in liquid nitrogen vapor tanks.
 
BAL Cryopreserved cells 
Cells are split into 10 million cells per aliquot. They are stored in cryogenic safe plastic boxes in liquid nitrogen vapor tanks.
 
BAL Fluid 
BALF samples are split into cryovials – 3.5 ml per vial. They are stored at -80 °C in the 2 inch fiberboard boxes.
 
Specific processing procedures and storage conditions can be found in the Beryllium BioBank Protocol:

 

 



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