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Department of Bioengineering



Research Publications 2012-current 

  • Thomas H. Hraha, Matthew J. Westacott, Marina Pozzoli, P. Mason McClatchey, Richard K.P. Benninger. “Phase Transitions in the Multi-cellular Regulatory Behavior of Pancreatic Islet Excitability” Manuscript in revision.

  • Nikki L. Farnsworth, Alireza Hemmati, Marina Pozzoli, Thomas H. Hraha, Richard K.P. Benninger. “Fluorescence Recovery After Photobleaching Reveals Regulation and Distribution of Cx36 Gap Junction Coupling within Mouse Islets of Langerhans” Manuscript in revision.​

    • Richard K.P. Benninger, W. Steven Head, Min Zhang, Troy Hutchens, Sylvain J. Le Marchand, Leslie S. Satin, David W. Piston. “Intrinsic islet heterogeneity and coupling determine spatio-temporal Ca wave dynamics: testing predictions and verification of a mathematical model of the islet” Manuscript in revision.​

  • Linda M. Nguyen, Marina Pozzoli, Thomas H. Hraha, Richard K.P. Benninger. “Decreasing Cx36 gap junction coupling compensates for overactive KATP channels to restore insulin secretion and prevent hyperglycemia in a mouse model of neonatal diabetes” Diabetes (2014) 63(5) pp1685-97.

  • Thomas H. Hraha, Abigail B. Bernard, Linda M Nguyen, Kristi S. Anseth, Richard K.P. Benninger. “Dimensionality and size scaling of coordinated Ca2+ dynamics in pancreatic β-cell clusters” Biophysical Journal (2014) 106 pp299-309. 

  • W. Steven Head, Meredith L. Orseth, Craig S. Nunemaker, Leslie S. Satin, David W. Piston, Richard K.P. Benninger. “Connexin-36 gap junctions regulate in-vivo first and second phase insulin secretion dynamics and glucose tolerance in the conscious mouse” Diabetes (2012)  6 pp1700-07. *Special Commentary.

 ​​Research Publications 2004-2011:
  • Richard K.P. Benninger, W. Steven Head, Min Zhang, Leslie S. Satin, David W. Piston. “Gap junctions act in combination with other mechanisms of cell-cell communication to supress basal inuslin secretion in the islet of Langerhans” Journal of Physiology (2011)  589 pp5453-66.​

  • ​​Richard K.P. Benninger, Maria S. Remedi, Alessandro Ustione, W. Steven Head, David W. Piston, Colin G. Nichols. “Defects in β-cell Ca2+ signalling and glucose metabolism underlie defective insulin secretion in a model of KATP channel-induced neonatal diabetes mellitus” Diabetologia (2011) 54 pp1087-97.
  • Aizhen Zhao, Yuhan Hao, Marcella Brissova, Richard K.P. Benninger, Joel Abramowitz, Guylain Boulay Alvin C. Powers, David W. Piston, Meisheng Jiang, Lutz Birnbaumer, Guoqiang Gu. “GαO represses insulin secretion by regulating insulin vesicular docking in pancreatic β cells” Diabetes (2010)  59 pp2522-9.
  • Michael Meyer-Hermann, Richard K. P. Benninger “A mathematical model of β-cells in an islet of Langerhans sensing a glucose gradient” HFSP Journal (2010)  4 pp61-71.
  • Lynley D. Pound, Suparna Sarkar, Richard K.P. Benninger, Yingda Wang, Adisak Suwanichkul, Melanie K. Shadoan, Richard L. Printz, James K. Oeser, Catherine E. Lee, David W. Piston, Owen P. McGuinness, John C. Hutton, David R. Powell, Richard M. O'Brien “Deletion of the Mouse Slc30a8 Gene Encoding Zinc Transporter-8 Results in Impaired Insulin Secretion” Biochemical Journal (2009)  421 pp371-376.
  • Christopher J. Easley, Richard K.P. Benninger, Jesse H. Shaver, W. Steven Head, David W. Piston   “Rapid and inexpensive fabrication of polymeric microfluidic devices via toner transfer masking” Lab on a Chip (2009) 9 pp1119-1127.

  • Subhadra C. Gunawardana, Richard K.P. Benninger, David W. Piston “Subcutaneous transplantation of embryonic pancreas for correction of type 1 diabetes” American Journal of Physiology- Endocrinology and Metabolism (2009) 296 E323-332.

  • Richard K.P. Benninger, Bruno Vanherberghen, Stephen Young, Sabrina Tanner, Fiona F. Culley, Tim Schnyder, Mark A.A. Neil, Daniel Wüstner, Paul M.W. French, Daniel M. Davis, Björn Önfelt “Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of Natural Killer cell immune synapses” Biophysical Journal- Biophysical Letters (2009) 96 L13-15. 

  • Richard K.P. Benninger, William J. Ashby, Elisabeth A. Ring, David W. Piston “A single-photon counting detector for increased sensitivity in two-photon laser scanning microscopy” Optics Letters (2008) 33: pp2895-7.

  • Richard K.P. Benninger, Min Zhang, W. Steven Head, Leslie S Satin, David W. Piston “Gap Junction Coupling and Calcium Waves in the Pancreatic Islet” Biophysical Journal (2008) 95: pp5048-5061. 

  • Richard K.P. Benninger*, Shu Mao*, Yuling Yan, Chutima Petchprayoon, David Jackson, Chistopher J. Easley, David W. Piston, Gerard Marriott “Optical lock-in detection of fluorescence resonance energy transfer using synthetic and genetically-encoded optical switches” Biophysical Journal (2008) 94: pp4515-24. (* Equal contributors)

  • Sunil Kumar, Christopher Dunsby, Pieter A.A. DeBeule, Dylan M. Owen, U Anand, Peter M.P. Lanigan, Richard K.P. Benninger, Daniel M. Davis, Mark A.A. Neil, Praveen Anand, Cristopher Benham, Alan Naylor, Paul M.W. French. “Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging” Optics Express. (2007) 15: pp12548-12561

  • Richard K.P. Benninger, Oliver Hoffman, Björn Önfelt, Ian Munro, Christopher Dunsby, Daniel M. Davis, Mark A.A. Neil, Paul M.W. French, Andrew J. deMello. “Fluorescence Lifetime Imaging of Dye: DNA Interactions within Continuous Flow Microfluidic Systems” Angewandte Chemie International Edition (English). (2007) 46: pp2228-2231

  • Pieter DeBeule, Dylan M. Owen, Hugh B. Manning, Clifford B. Talbot, Jose Requejo-Isidro, Christopher Dunsby, James McGinty, Richard K.P. Benninger, Daniel Elson, Ian Munro, M. John Lever, Pravene Anand, Mark A.A. Neil, Paul M.W. French.  “Rapid Hyperspectral Fluorescence Lifetime Imaging” Microscopy Research Techniques (2007) 70: pp481-484

  • Björn Önfelt, Shlomo Nedvetski, Richard K.P. Benninger, Mark A Perbhoo, Stefanie Sowinski, Alistair N. Hume, Miguel C. Seabra, Mark A.A. Neil, Paul M.W. French, Daniel M. Davis. “Structurally Distinct Membrane Nanotubes between Human Macrophages Support Long-Distance Vesicular Traffic or Surfing of Bacteria” Journal Immunology (2006) 177: pp8476-83.

  • Richard K.P. Benninger, Yasemin Koç, Oliver Hoffman, Jose Requejo-Isidro, Mark A.A. Neil, Paul M.W. French, Andrew J. deMello. “Quantitative 3D Mapping of Fluidic Temperatures within Microchannel Networks Using Fluorescence Lifetime Imaging” Analytical. Chemistry (2006) 78: pp2272-2278

  • Richard K.P. Benninger, Oliver Hoffman, James McGinty, Jose Requejo-Isidro, Ian Munro, Mark A.A. Neil, Andrew J. deMello, Paul M.W. French. “Time-resolved fluorescence imaging of solvent interactions in microfluidic devices” Optics Express. (2006) 13: pp6275-6285.

  • Richard K.P. Benninger, Björn Önfelt, Mark A.A. Neil, Daniel M. Davis, Paul M.W. French. “Fluorescence Imaging of Two-Photon Linear Dichroism: Cholesterol Depletion disrupts Molecular Orientation in Cell Membranes.” Biophysical Journal (2005) 88: pp609-622.

Review Articles and Book Chapters 

  • David W. Piston, Richard K.P. Benninger. “Cellular Communication and Heterogeneity in the Dynamics of Pancreatic Islet Insulin Secretion” Trends in Endocrinology and Metabolism. (2013)  Invited Review, In press.​

  • Nikki L. Farnsworth, Richard K.P. Benninger. “New insights into the role of connexins in pancreatic islet function and diabetes” FEBS Letters. (2014) 588(8) pp1278-1287, Invited Review.

  • Richard K.P. Benninger “Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order” Methods in Membrane Lipids, (2013 Invited Review, In Press.  

  • Richard K.P. Benninger and David W. Piston “Two-Photon Excitation Microscopy for the Study of Living Cells, Tissue and In-vivo” Current Protocols in Cell Biology, (2013) 59:4.11.1-4.11.24.

  • David W. Piston, Gert-Jan Kremers, Richard K.P. Benninger, and Michael W. Davidson  “Photoactivation in Fluorescence Microscopy” Microsocpy Today (2009) 17 pp8-13.

  • Richard K.P. Benninger, David W Piston. “Recent Advances in Single-photon-counting Detection Technology in Fluorescence Microscopy” Laser Focus World. (2009) June: pp59-63.

  • Richard K.P. Benninger, Mingming Hao, David W. Piston. “Multi-photon Excitation Imaging of Dynamic Processes in Living Cells and Tissues” Reviews of Physiology, Biochemistry and Pharmacology (2008) 160: pp71-92.

  • James McGinty, Christopher Dunsby, Egidijus Auksorius, Richard K.P. Benninger, Pieter De Beule, Daniel S. Elson, Neil Galletly, David Grant, Oliver Hofmann, Gordon Kennedy, Sunil Kumar, Peter M. P. Lanigan, Hugh Manning, Ian Munro, Björn Önfelt, Dylan Owen, Jose Requejo-Isidro, Klaus Suhling, Clifford B. Talbot, P. Soutter, M. John Lever, Andrew J. deMello, Gordon S. Stamp, Mark A.A. Neil, and Paul M.W. French “Multidimensional fluorescence imaging” Laboratory Techniques in Biochemistry and Molecular Biology 33 (2008)  Ed. T.W.J. Gadella

  • Daniel S. Elson, Niel Galletly, Clifford Talbot, Jose Requejo-Isidro, James McGinty, Christopher Dunsby, Peter M.P. Lanigan, Ian Munro, Richard K.P. Benninger, Pieter DeBeule, Egidijus Auksorius, Laszlo Hegyi, Anne Sandison, Andrew Wallace, Pat Soutter, Mark A.A. Neil, John Lever, Gordon W. Stamp, Paul M.W. French. “Multidimensional Fluorescence Imaging Applied to Biological Tissue” Reviews in Fluorescence. (2006)  Ed. C.D. Geddes and J.R. Lakowicz

  • Daniel Elson, Jose Requejo-Isidro, Ian Munro, Fred Reavell, Jan Siegel, Klaus Suhling, Paul Tadrous, Richard Benninger, Peter Lanigan, James McGinty, Clifford Talbot, Bebhinn Treanor, Stephen Webb, Anne Sandison, Andrew Wallace, Daniel Davis, John Lever, Mark Neil, David Phillips, Gordon Stamp, Paul French. “A Perspective: Time-domain fluorescence lifetime imaging applied to biological tissue.” Photochemical Photobiological Sciences (2004) 3: pp795–801