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University of Colorado Denver College of Liberal Arts and Sciences

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Faculty & Staff Directory

Christopher Phiel, Ph.D.


Dr. Christopher Phiel

Assistant Professor

Email:  christopher.phiel@ucdenver.edu

Office Location:   SI 4111

Phone:   (303) 556-5681

Fax: (303) 556-4352

​2015 Spring Semester Office Hours:
By Appointment Only

Areas of Expertise: Molecular Biology

Lab website: http://claslabs.ucdenver.edu/chris-phiel-lab/​

Education

1998 Ph.D. Developmental Biology
Thomas Jefferson University, Philadelphia, PA

 

1992 B.S. Biology
Ursinus College, Collegeville, PA

 

There are two main interests in our lab. First, we are interested in understanding the various biological functions regulated by glycogen synthase kinase-3 (Gsk-3) isoforms. We are utilizing a systems biology approach to understanding Gsk-3 function in embryonic stem cells, neuronal cells, mice, and humans. These studies have shown that Gsk-3 activity is important for regulating DNA methylation, and we are very interested in learning more about how signal transduction affects epigenetics.

Publications

Bartman, C.M., Egelston, J., Ren, X., Das, R., and Phiel, C.J. (2015) A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine, Experimental Cell Research, 330: 178-185. http://dx.doi.org/10.1016/j.texcr.2014.07.020, PMID: 25102378.

Meredith, G., D’Ippolito, A., Zeidner. L.C., Hostetter, L., Popkie, A.P., Doble, B.W., Marnellos, G., Adams, C., Wang, Y., and Phiel, C.J. (2015) Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells, Submitted, Molecular Biology of the Cell.

Battacharjee, R., Dudki, T., Goswami, S., Popkie, A., Phiel, C.J., Klein, D., and Vijayaraghavan, S. (2015) Targeted disruption of GSK-3α affects sperm motility resulting in male infertility, Accepted, Biology of Reproduction.

Bartman, C.M., Egelston, J., Zeidner, L.C., D’Ippolito, A., Kattula, S., Doble, B.W. and Phiel, C.J. (2014) Gene expression profiling in mouse embryonic stem cells reveals glycogen synthase kinase-3-dependent targets of phosphatidylinositol 3-kinase and Wnt/ß-catenin signaling pathways, Frontiers in Endocrinology, 5:133. doi:10.3389/fendo.2014.00133, eCollection 2014. PMID: 25165462.

Zhen, C.Y., Duc, H.N., Kokotovic, M., Phiel, C.J., and Ren, X. (2014) Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism, Accepted, Molecular Biology of the Cell 25 (23):3726-3739. mbc.E14-06-1109, PMID: 25232004.

D’Ippolito, A., Morii, Y., Bartman, C.M., Zeidner, L.C., and Phiel, C.J. (2014) Functionally Redundant Roles of Dnmt3a and Dnmt3b in the Catalysis of Non-CpG DNA Methylation at the Sf1 Locus in Mouse Embryonic Stem Cells, Submitted, Journal of Stem Cells Research, Development and Therapy.

Bartman, C.M., Egelston, J., Ren, X., Das, R., and Phiel, C.J. (2015) A Simple and Efficient Method for Transfecting Mouse Embryonic Stem Cells Using Polyethylenimine, Experimental Cell Research, 330: 178-185. http://dx.doi.org/10.1016/j.texcr.2014.07.020, PMID: 25102378.

Meredith, G., D’Ippolito, A., Zeidner. L.C., Hostetter, L., Popkie, A.P., Doble, B.W., Marnellos, G., Adams, C., Wang, Y., and Phiel, C.J. (2015) Glycogen Synthase Kinase-3 (Gsk-3) Plays A Fundamental Role In Maintaining DNA Methylation At Imprinted Loci In Mouse Embryonic Stem Cells, Submitted, Molecular Biology of the Cell.

Battacharjee, R., Dudki, T., Goswami, S., Popkie, A., Phiel, C.J., Klein, D., and Vijayaraghavan, S. (2015) Targeted disruption of GSK-3α affects sperm motility resulting in male infertility, Accepted, Biology of Reproduction.

Bartman, C.M., Egelston, J., Zeidner, L.C., D’Ippolito, A., Kattula, S., Doble, B.W. and Phiel, C.J. (2014) Gene expression profiling in mouse embryonic stem cells reveals glycogen synthase kinase-3-dependent targets of phosphatidylinositol 3-kinase and Wnt/ß-catenin signaling pathways, Frontiers in Endocrinology, 5:133. doi:10.3389/fendo.2014.00133, eCollection 2014. PMID: 25165462.

Zhen, C.Y., Duc, H.N., Kokotovic, M., Phiel, C.J., and Ren, X. (2014) Immobilization of PRC1 Complex at Mitotic Chromosomes by Cbx2 in a PRC2-Independent Mechanism, Accepted, Molecular Biology of the Cell 25 (23):3726-3739. mbc.E14-06-1109, PMID: 25232004.

D’Ippolito, A., Morii, Y., Bartman, C.M., Zeidner, L.C., and Phiel, C.J. (2014) Functionally Redundant Roles of Dnmt3a and Dnmt3b in the Catalysis of Non-CpG DNA Methylation at the Sf1 Locus in Mouse Embryonic Stem Cells, Submitted, Journal of Stem Cells Research, Development and Therapy.

Phiel, C.J., Wilson, C.A., Lee, V.M.-Y., and Klein, P.S. (2011) Reply to GSK-3α/β kinases and amyloid production in vivo, Nature, 480:E6. PMID: 22158251.

Zeidner, L.C., Buescher, J.L., and Phiel, C.J. (2011) A Novel Interaction Between Glycogen Synthase Kinase-3α (GSK-3α) and the Scaffold Protein Receptor for Activated C-Kinase 1 (RACK1) Regulates the Circadian Clock, International Journal of Biochemistry and Molecular Biology, 2(4):318-327. PMID: 22187666.

Fleming, J.L., Phiel, C.J., and Toland, A.E. (2011) The Role for Oxidative Stress in Aberrant DNA Methylation in Alzheimer’s Disease, Current Alzheimer Research, In Press. PMID: 21605062.

Popkie, A.P., Zeidner, L.C., Albrecht, A.M., D’Ippolito, A., Eckardt, S., Newsom, D.E., Groden, J., Doble, B.W., Aronow, B., McLaughlin, K.J., White, P., and Phiel, C.J. (2010) Phosphatidylinositol 3-Kinase (PI3K) Signaling via Glycogen Synthase Kinase-3 (Gsk-3) Regulates DNA Methylation of Imprinted Loci, Journal of Biological Chemistry, 285: 41337-41347. PMID: 21047779.

Buescher, J.L. and Phiel, C.J. (2010) A Noncatalytic Domain of GSK-3 Is Essential for Activity, Journal of Biological Chemistry, 285:7957-7963. PMID: 20080974.

He, F., Popkie, A.P., Xiong, W., Li, L., Phiel, C.J.*, and Chen, Y. (2010) Gsk-3β is Required in the Epithelium for Palatal Elevation in Mice, Developmental Dynamics, 239:3235-3246. PMID: 20981831 (*Co-corresponding author).

Yang, J. and Phiel, C.J. (2010) Functions of B56-Containing PP2As in Major Developmental and Cancer Pathways, Life Sciences, 87:659-666. PMID:20934435.

Rorick, A.M., Mei, W., Liette, N.L., Phiel, C.J., El-Hodiri, H., and Yang, J. (2007) PP2A:B56e is Required For Eye Induction and Eye Field Separation, Developmental Biology, 302:477-493. PMID: 17074314.

Checler, F., Alves da Costa, C., Ayral, E., Andrau, D., Dumanchin, C., Farzan, M., Hernandez, J.F., Martinez, J., Lefranc-Jullien, S., Marambaud, P., Pasini, A., Petit, A., Phiel, C.J., Robert, P., St. George-Hyslop, P., and Wilk, S. (2005) JLK Inhibitors: Isocoumarin Compounds as Putative Probes to Selectively Target the γ-Secretase Pathway, Current Alzheimer Research, 2:327-334. PMID: 15974898.

Phiel, C.J., Wilson, C.A., Lee, V.M.-Y., and Klein, P.S. (2003) GSK-3α Regulates Production of Alzheimer’s Disease Amyloid-β Peptides, Nature, 423:435-439. PMID: 12761548.

Zhang, F., Phiel, C.J., Spece, L., Gurvich, N., and Klein, P.S. (2003) Inhibitory Phosphorylation of Glycogen Synthase Kinase-3 (GSK-3) in Response to Lithium: Evidence for Autoregulation of GSK-3, Journal of Biological Chemistry, 278:33067-33077. PMID: 12796505.

Checler, F., Pasini, A., Alves da Costa, C., Ayral, E., Hernandez, J.F., Dumanchin-Njock, Phiel, C.J., Marambaud, P., Wilk, S., Farzan, M., Boisbrun, M., Fulcrand, P., Martinez, J., Andrau, D., and Petit, A. (2003) JLK isocoumarin inhibitors: Selective γ-Secretase Inhibitors That Do Not Interfere With Notch Pathway In Vitro or In Vivo, Journal of Neuroscience Research, 74:370-377. PMID: 14598313.

Gabetta, V., Trzyna, W.T., Phiel, C.J., and McHugh, K.M. (2003) Vesicle-Associated Protein-A is Differentially Expressed During Intestinal Smooth Muscle Cell Differentiation, Developmental Dynamics, 228:11-20. PMID: 12950075.

Phiel, C.J., Zhang, F., Huang, E.Y., Guenther, M.G., Lazar, M.A., and Klein, P.S. (2001) Histone Deacetylase is a Direct Target of Valproic Acid, a Potent Anticonvulsant, Mood Stabilizer, and Teratogen, Journal of Biological Chemistry, 276:36734-36741. PMID: 11473107.

Phiel, C.J. and Klein, P.S. (2001) Molecular Targets of Lithium Action, Annual Reviews in Pharmacology and Toxicology, 41:789-813. PMID: 11264477.

Phiel, C.J., Gabbeta, V., Parsons, L.M., Rothblat, D., Harvey, R.P., and McHugh, K.M. (2001) Differential Binding of an SRF/NK-2 Transcription Factor Complex in Normal Versus Neoplastic Smooth Muscle Tissues, Journal of Biological Chemistry, 276:34637-34650. PMID: 11457859.

Brittingham, J.W. *, Phiel, C.J. *, Trzyna, W.T. *, Gabbeta, V., and McHugh, K.M. (1998) Identification of Distinct Molecular Phenotypes in Cultured Gastrointestinal Smooth Muscle Cells. Gastroenterology 115:605-617. PMID: 9721158. (*Authors contributed equally).

BIOL 3124 Introduction to Molecular Biology
BIOL 4125/5125 Molecular Biology Lab
​BIOL 4128/5128 Topics In Molecular Biology​