Christopher J. Phiel
, PhD, assistant professor, in the College of Liberal Arts and Sciences, Department of Integrative Biology, recently has had two papers accepted for publication.
The first paper is a multi-disciplinary effort by Phiel and colleagues in the Department of Integrative Biology including Professional Research Assistant Colleen Bartman, MS; student Jennifer Egelston; Raibatak Das, PhD; as well as Xiaojun Ren, PhD, Chemistry.
The paper describes the development of a new protocol for introducing DNA into mouse embryonic stem cells, a widely-used cell type in academic and industry laboratories.
Phiel explained that the researchers new method is comparable to existing methods, but is substantially cheaper – for the same price of a commercially available reagent that can be used for 250 reactions, they can perform over 82,000 reactions. He said they hope this new method will allow laboratories working with stem cells to stretch their research dollars.
The second paper is entitled “Gene Expression Profiling in Mouse Embryonic Stem Cells Reveals Gsk-3-Dependent Targets of PI3K and Wnt/β-Catenin Signaling Pathways." It was accepted for publication at Frontiers in Endocrinology.
This work provides detailed comparisons of the expression of all genes in different mouse embryonic stem cells in which Gsk-3 activity has been inhibited or genetically deleted. These results provide a broader perspective on how Gsk-3 activity affects gene expression.
Phiel also recently was recognized with a CLAS CRISP award and an ORDE Large Grant award.
His research focuses on signal transduction and epigenetics in the context of neurological diseases.