Results could have worldwide implications and prove that all lab mice are not equal
AURORA, Colo. (June 29, 2010) – Researchers at theUniversity of Colorado’s Anschutz Medical Campus have found the brains of mice used in laboratories worldwide can be profoundly affected by the type of cage they are kept in, a breakthrough that may require scientists to reevaluate the way they conduct future experiments.
“We assume that mice used in laboratories are all the same, but they are not,” said Diego Restrepo, director of the Neuroscience Program and professor of cell and developmental biology whose paper on the subject was published Tuesday, June 29. “When you change the cages you change the brains and that affects the outcomes of research.”
Mice are the chief research mammals in the world today with some of the most promising cancer, genetic and neuroscience breakthroughs riding on the rodents. Researchers from different universities rely on careful comparison of experimental results for their discoveries; but Restrepo has found that some of these comparisons may not be trustworthy.
He discovered that the brains of mice are extremely sensitive to their environment and can physically change when moved from an enclosure where air circulates freely to one where it doesn’t. Specifically, the portion of the mouse’s brain responsible for its keen sense of smell, the olfactory bulb, is altered. Restrepo also found profound changes in the levels of aggression when mice are moved from one type of cage to another.
The results, he says, can greatly affect the accuracy of the research. Two labs doing the same experiments may get totally different results and never know why.
“This could explain some of the failures to replicate findings in different laboratories and why contradictory data are published by different laboratories even when genetically identical mice are used as subjects,” said Restrepo.
The consequences could mean good science derailed or promising research abandoned simply due to the design of a mouse cage – something largely overlooked until now.
Restrepo’s findings were just published in PLoS One, the Public Library of Science, a major peer-reviewed scientific journal, and are gaining and increasingly wide audience.
He hopes scientists will work to uncover the depth of the problem and find ways to overcome it.
"We need to ensure that laboratory findings are truly indicative of natural processes and not simply the result of environmental factors within each lab,” he said.
About the Anschutz Medical Campus
The Anschutz Medical Campus is the largest academic health center between Chicago, Texas and the West Coast. The campus is home to the health sciences programs of the University of Colorado as well as University of Colorado Hospital, the University of Colorado Cancer Center and the Barbara Davis Center for Childhood Diabetes. The University of Colorado Anschutz Medical Campus researchers have a proven record of success and expertise in innovation, discovery and commercialization of therapies, drugs and medical devices. Research accomplishments of being “the first” include the development of a classification and numbering system for human chromosomes, the identification of a genetic factor that converts normal cells into cancer cells, discovering that lymphocytes are preprogrammed to respond to antigens, the foundation of modern immunology, how a human cancer gene functions, and first to identify that naturally occurring proteins in the blood prevent the AIDS virus from reproducing and spreading to healthy cells. The Anschutz Medical Campus stands as a model across the nation for a successful redevelopment of a decommissioned army base.
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