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Assays and methods used in the laboratory:


The Hirsch Laboratory is CLIA certified #06D2003207.

               

Protein Assessment:  Conventional IHC and AQUA (automated fluorescence labeled assessment.  In collaboration with HistoRx).

 

Gene Copy Number:  The lab is doing Silver In-Situ Hybridization (SISH) in collaboration with Ventana-Roche and doing Fluorescence In-Situ Hybridization (FISH) studies in collaboration with the University of Colorado Cancer Center (UCCC) Cytogenic Core.   (Director:  Marileila Varella Garcia, Ph.D.).

The lab is also working closely with UCCC Pathology Core (Director:  Wilbur Franklin, M.D.) regarding mutation analysis.

 

MicroRNA assays:  Relative quantitative assessment of microRNA expression (individual assay and panel multiplex assays) by taqman qRT-PCR after nucleic acid extraction from fresh frozen tissue and from formalin fixed, paraffin embedded tissue, both from tumor and from small bronchial biopsies.